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Name: Jenny Yeh and Mary Catherine Wen

Name: Jenny Yeh and Mary Catherine Wen High School: St. Agnes Academy and Archbishop Molloy High School Mentor: Dr. Miriam Rafailovich Project Title: Proliferation and Alignment of Osteoblasts on Oriented Magnetic Nanocomposites.

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Name: Jenny Yeh and Mary Catherine Wen

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  1. Name: Jenny Yeh and Mary Catherine Wen • High School: St. Agnes Academyand Archbishop Molloy High School • Mentor: Dr. Miriam Rafailovich • Project Title: Proliferation and Alignment of Osteoblasts on Oriented Magnetic Nanocomposites • Tissue engineering to improve cell proliferation and alignment of osteoblasts on scaffolds for bone grafts can facilitate the healing of fractures and nonunions. Research has shown that strong magnetic loads can stimulate bone formation and orientation along the field, and studies have shown that mimicking the topography of an extracellular matrix, the native environment for a cell, with an electrospun scaffold enhances the structure and adaptation of the tissue. Magnetic substrates have also been exposed to external magnetic fields for biomedical applications such as biosensors, targeted drug delivery, and artificial implants. Combining these aspects, MC3T3 osteoblasts were plated on magnetic and diamagnetic surfaces of different topologies and cultured both with and without an external magnetic field. Four surfaces were created, 100% EVA 260, 90% EVA 260 with 10% Cloisite 20A, 90% EVA 260 with 10% Cloisite 20A:Iron Pentacarbonyl in a 1:1 ratio by mass, and 90% EVA 260 with 10% Cloisite 20A:Iron Pentacarbonyl in a 1:2 ratio by mass, and formed into three topographies: bulk disks, electrospun mesh fibers, and electrospun aligned fibers. Vibrating Sample Magnetometry, Fourier Transform Infrared Spectroscopy, Thermogravitometric Analysis, and Dynamic Mechanical Analysis determined the mechanical and magnetic characteristics of the materials. Cell counts and confocal microscopy investigated the proliferation and alignment of the osteoblasts on the magnetic nanocomposites. The study indicated that a biologically and mechanically compatible superparamagnetic nanocomposite was created from electrospun aligned fibers of 90% EVA 260 with 10% Closite 20A:Iron Pentacarbonyl in a 1:2 ratio cultured in a magnetic field with the potential to accelerate fracture healing and nonunions.

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