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Synthetic Biology - Progess in Merging Nanoparticles and Proteins

Synthetic Biology - Progess in Merging Nanoparticles and Proteins. Kie Moon Sung David Mosley Joseph Jacobson Center for Bits and Atoms, MIT Beau Peele Shuguang Zhang Biomedical Engineering Dept, MIT. Nanoparticle Building Blocks. COOH. COOH. NH 2. NH 2. COOH. COOH. NH 2. NH 2.

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Synthetic Biology - Progess in Merging Nanoparticles and Proteins

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  1. Synthetic Biology - Progess in Merging Nanoparticles and Proteins Kie Moon Sung David Mosley Joseph Jacobson Center for Bits and Atoms, MIT Beau Peele Shuguang Zhang Biomedical Engineering Dept, MIT

  2. Nanoparticle Building Blocks COOH COOH NH2 NH2 COOH COOH NH2 NH2 COOH NH2 Natural amino acids Amino acid-nanoparticles

  3. Synthesis of Lys-Monofunctionalized Gold Nanocrystals aReagents and conditions: (a) H2NNH2·H2O/DMF (2% v/v), DIPEA/DMF (10% v/v). (b) HS(CH2)10CO2H (excess), THF. (c) DIC (excess), DMF/THF (10% v/v) rt, 24h. (d) MeNH2, DIC (excess), DMF/THF, rt, 12h. (e) 60% TFA, 2.5% TIS, 2.5% H2O, 35% DMF, 24h.

  4. Identification of Monofunctionalized Nanocrystals Nanocrystal Dimers 10 nm At least 60% of Nanocrystals are monofunctionalized. J.Am.Chem. Soc., 126 (2004), p. 5064

  5. Programmable Assembly of Nanocrystal-Peptides Fmoc SPPS Protocols peptide1 peptide2 peptide3

  6. Ultimate Goals • New Structures - New peptide-folding rules governed by nanoparticles. • New Functions – Electronic control of specific protein or enzyme functions, electronic feedback aka new biosensor techniques. • New materials – Both bioinorganics and a new class of nanoparticle polymers. All aided by the programmable assembly nature of this building block based technology. This concept will allow us to access completely new motifs, and readily explore the phase space.

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