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Frequency Domain Optical Coherence Tomography (FDOCT)

Frequency Domain Optical Coherence Tomography (FDOCT). Joon S Kim IMSURE Summer Research Fellow At Beckman Laser Institute University of California at Irvine Irvine, CA 92612 Email: jkim627@mail.umd.edu. OCT. Optical Coherence Tomography

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Frequency Domain Optical Coherence Tomography (FDOCT)

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  1. Frequency Domain Optical Coherence Tomography (FDOCT) Joon S Kim IMSURE Summer Research Fellow At Beckman Laser Institute University of California at Irvine Irvine, CA 92612 Email: jkim627@mail.umd.edu

  2. OCT • Optical Coherence Tomography • New field of imaging technology that can complement conventional imaging techniques • Based on interference of EM wave • Provide high spatial resolution cross-sectional view of tissues without excision • Endless potential biomedical applications • Non-invasive Diagnostics

  3. Resolution (log) 1 mm Ultrasound 100 mm 10 mm Confocalmicroscopy 1 mm Penetration depth (log) 1 mm 1 cm 10 cm OCT vs. Conventional Imag. Tech Standardclinical Highfrequency OCT

  4. Two kinds of OCT • TDOCT • Time Domain OCT • Use of Scanning Mirror • FDOCT • Fourier (Frequency) Domain OCT • Use of laser source over band of optical frequency • Advantages over TDOCT

  5. My research experience with FDOCT are in 2 phases… • First phase: • Understanding and Replicating FDOCT experimental setting from scratch • Second phase: • Learning the physical and mathematical reasoning behind the magical DSP algorithm which extract images out of OCT data!

  6. Part 1 Swept source Mirror • FDOCT experiment set up Grating Collimator 2 x 2 Coupler Phase Modulator Attenuator Fiber Fabry-Perot interferometer Probe Detector1 Sample Detector2

  7. Current FDOCT System • No human intervention required • Everything controlled from execution module in host computer • Host computer • houses NI-6112: A2D Conversion of OCT data • performs digital signal processing • controls peripherals

  8. Scanning sample • Surface of sample is divided into imaginary grid. • Each block in grid: Pixel • Scanning in X direction • Lateral scan • Scanning in Z direction • A scan Z Y X

  9. Scanning sample (cont.) • 1600 samples / A-scan (pixel) • 400 A-scan / Lateral scan • Lateral scanning Frequency: 2 KHz • A-scanning Frequency: 10 MHz • Limited by A2D converter (NI-6112) • Pixel size: 10 micron • Slow process => not applicable in Vivo

  10. With NI-5122 • Max. Sampling Frequency: 100 MS/sec • A-scanning Frequency: 100 MHz • More time for DSP • Boost lateral scanning frequency to 20 KHz • One step closer to in Vivo application of FDOCT

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