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2004. 5. 25 Ji-Yoon Park

Nanoparticle-Based Theorem Proving. 2004. 5. 25 Ji-Yoon Park. Target Problem. P ∧Q→R, S∧T→Q, S, T, P R = True ?. DNA-Based Nanoparticle Assembly Strategy. Mirkin, 2000. Experimental Section. 1. Purchase of gold nanoparticle.

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2004. 5. 25 Ji-Yoon Park

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  1. Nanoparticle-Based Theorem Proving 2004. 5. 25 Ji-Yoon Park

  2. Target Problem P∧Q→R, S∧T→Q, S, T, P R = True ?

  3. DNA-Based Nanoparticle Assembly Strategy Mirkin, 2000

  4. Experimental Section 1. Purchase of gold nanoparticle 2. Preparation of 3’-(alkanethiol) modified oligonucleotides 3. Preparation of Linker DNA 4. Characterization of DNA-linked gold assembly

  5. The Sequence of Oligonucleotides

  6. 45 mer 15 mer 15 mer Hypothesis S1 Digestion Assembly

  7. Colloidal Gold

  8. Full Assembly vs S1 Digestion

  9. Partial Assembly vs S1 Digestion

  10. TEM Image (Colloidal Gold) (a) (b) (c) TEM image of the annealed DNA-linked assembly (a) Colloidal gold (b) Full-assembly of DNA-linked nanoparticles (c) S1 Nuclease digestion of full-assembled DNA-linked nanoparticles Figure 2. TEM image of the annealed DNA-linked assembly (a) Colloidal gold (b) Full-assembly of DNA-linked nanoparticles (c) S1 Nuclease digestion of full-assembled DNA-linked nanoparticles Figure 2. TEM image of the annealed DNA-linked assembly (a) Colloidal gold (b) Full-assembly of DNA-linked nanoparticles (c) S1 Nuclease digestion of full-assembled DNA-linked nanoparticles Figure 2. TEM image of the annealed DNA-linked assembly (a) Colloidal gold (b) Full-assembly of DNA-linked nanoparticles (c) S1 Nuclease digestion of full-assembled DNA-linked nanoparticles

  11. Surface-Based Theorem Proving

  12. Surface-based DNA Computation Liu et al., 2000

  13. Experimental Section 1. Purchase of gold nanoparticle 2. Preparation of 3’-(alkanethiol) modified oligonucleotides 3. Preparation of Linker DNA 4. Characterization of DNA-linked gold assembly

  14. ¬RPQ ¬RPQ ¬RPQ R ¬RPQ ¬RPQ ¬RPQ ¬T ¬T ¬T ¬P ¬T ¬T ¬T ¬S ¬S ¬S ¬S ¬S ¬S ST ¬Q P.C P.C P.C P.C P.C P.C N.C N.C N.C N.C N.C N.C Theorem Proving by Fluorescence Imaging A B C A B C (b) (a) I II III IV V I II III IV V A B C I II III IV V (c)

  15. Further Work • Paper publish • Genotyping experiment • Structure & Theorem Proving

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