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FERTILISASI

FERTILISASI. PROBLEMS. How does the sperm head enter the egg? How does the fertility events? How does the egg PREVENT entry of MORE THAN ONE sperm? (Polyspermy). Fusi. Sperma. Telur. Fusi. Sperma. Sperma. Polysperma. Fusi. Fusi. Telur. Fusi. Embrio. Sperma. Fertilization.

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FERTILISASI

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  1. FERTILISASI

  2. PROBLEMS How does the sperm head enter the egg? How does the fertility events? How does the egg PREVENT entry of MORE THAN ONE sperm? (Polyspermy)

  3. Fusi Sperma Telur Fusi Sperma Sperma Polysperma Fusi Fusi Telur Fusi Embrio Sperma Fertilization

  4. Fertilisasi Kromosom (n) dari 2 individu 1 sel diploid (2n) Telur teraktivasi Zigot Stimulasi meiosis Perkembangan embrio Blok sperma masuk

  5. TAHAPAN FERTILISASI • Sperm Capacitation • Sperm-Zona Pellucida Binding • The Acrosome Reaction • Penetration of the Zona Pellucida • Sperm-Oocyte Binding • Egg Activation and the Cortical Reaction • The Zona Reaction

  6. Sperm Capacitation Fresh ejaculate Fertil ? Kapasitasi Fertil Perubahan pd Protein seminal Plasma & reorganisasi Lipid dan Protein membran sperma, Influx Ca,cAMP , pH intrasel  Motilitas hiperaktif Transpot gamet Inkubasi di media fertilisasi Bbrp jam

  7. Zona pellusida (ZP) Sperma Binding Sperm-Zona Pellucida Binding Interaksi receptor-ligand (species specificity) Kelp KH (glikoprotein) di ZP berfungsi seperti receptor sperma; stimulasi fusi membran plasma dg membran akrosom luar Receptor sperma → bbrpmcm protein

  8. The Acrosome Reaction Bind Sperma → ZP Fertilisasi Penetrasi ZP → Oosit Reaksi akrosom Lisosom (enzim digesti), anterior kepala sperma Akrosom sp → hilang Fusi sp-zp → sukses Integritas akrosom → analisis sperma

  9. Skema: Acrosome Reaction

  10. Zona pellusida (ZP) Sperma Menerobos Penetrasi Zona Pellucida • Keberhasilan penetrasi menembus ZP dipengaruhi oleh: • kekuatan ekor sperma (motilitas) • kombinasi enzim akrosomal

  11. Sperm-Oocyte Binding Membran oosit ZP Binds & Fuses Sperma • * Proses binding terjadi pd bag posterior (post-acrosomal) • daerah kepala sperma • * Molekul sperma yang berperan dlm proses tsb → glikoprotein • : Fertilin • * Peran fertilin: mengilat membran plasma oosit dan induksi fusi

  12. Oosit mature Fertilisasi Egg Metafase Sperma Aktivasi telur & Reaksi Cortical egg activation • Faktor yang mempengaruhi: • Konsentrasi Ca  tinggi • Kelengkapan meiosis II • Cortical reaction→ eksositosis, granula kortikal tampak pendek stlh fusi sperma-oosit. • Granula kortikal menghslkan bbrp enzim al. protease → difusi dlm ZP diikuti eksositosis dr telur dan mengubah struktur ZP (zona reaction). Komponen granula kartikal juga berinteraksi dgn membran plasma oosit.

  13. The Zona Reaction Reaksi zona Struktur ZP Blok polisperma Katalis protease ZP mengeraskan Rusak reseptor Sperma di ZP Hambat sperma Masuk ZP Infertil

  14. File:Polyspermy If two or more sperm make it into the egg polyspermy occurs. In this case some of the genetic material condensed into chromosome is attracted to the extra sperm causing abnormal development and ultimately the death of the embryo. Too many sperm and toxic conditions can lead to polyspermy. Polyspermy is a significant problem for in vitro fertilization of humans (IVF). The polyspermy rate in IVF is about 10%.

  15. THANK YOU FOR YOUR ATTENTION

  16. 1. Kapan spermatozoa dikatakansiapuntukfertilisasi? 2. Padasaatfertilisasi, meiosis yang terjadipada ovum padafaseapa? 3. Kapan meiosis II dari ovum berakhir? 4. Apa yang dimaksuddengankapasitasi spermatozoa? 5. Apakahreaksiakrosomitu? 6. Enzimapasaja yang berperandalamreaksiakrosom? 7. Apa yang dimaksuddenganpenetrasizonapelusida? 8. Apa yang dimaksudpolispermia? 9. Sebutkan 2 alasan, mengapapolispermiadihindari? 10. Padasaatapapolispermiaseringterjadi?

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