1 / 24

指導人 : 褚俊傑 ( 老師 ) 鄭 秀 娟 (TA)

Optimization Of Lipase Production Media Parameters By A Newly isolated BACILLUS LICHENIFORMS KDP From Oil Mill Soil (impact factor:2.958). 指導人 : 褚俊傑 ( 老師 ) 鄭 秀 娟 (TA) 組員 : 鄭育軒 張弘儒 方嘉篁. Abstract.

traci
Télécharger la présentation

指導人 : 褚俊傑 ( 老師 ) 鄭 秀 娟 (TA)

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Optimization Of Lipase Production Media Parameters By A Newly isolated BACILLUS LICHENIFORMS KDP From Oil Mill Soil(impact factor:2.958) 指導人:褚俊傑(老師)鄭秀娟(TA) 組員:鄭育軒 張弘儒 方嘉篁

  2. Abstract To maximize the lipase enzymeproduction: lipase enzyme using various supplements along with theoptimal physical conditions for enhancing theproduction. Selenium(硒) is used as a media supplement and thus reported by us for the first time. The maximum lipase production (16.23U/ml) was found to be at 60μM/l. The maximum metal ion and nickel production was found to be 80μM/L.

  3. The new isolate produced their maximum extracellular lipase at pH 7.5 (18.69 U/mL) and 45°C (17.36U/mL). The maximum lipase yield 12.43U/mL and15.38U/mL was found to be in the medium containing 0.1mg/l urea and 1mg/l ammonium nitrate respectively. All the experiments proved that the physicochemical parameters and the lipase production are interdependent.

  4. Introduction Lipase的功用 Lipaseshave promising applications in organic chemical processing, detergent formulation, synthesis of biosurfactants, the agrochemical industry, waste water treatment, leather (removal of lipids from animal skins), medicine (blood triglyceride assay), paper manufacture, nutrition, cosmetics and pharmaceutical processing.

  5. Lipase Lipase production by microorganisms is highly influenced by medium components like nitrogen sources, carbon sources… • fatty acids • triglycerides • sugars • complex polysaccharides which can stimulate or repress lipase production.

  6. Lipase The most useful lipase producer genus used in industry is Bacillus among gram-positive bacteria. Bacillus subtilis Bacterium Bacillus subtilisits capability to secrete homologous and heterologous proteins in appreciable quantities into the growth medium.

  7. Methods(利用Extracellular lipase assay) • Samples of the culture medium were withdrawnand centrifuged for 15 min at 10,000g.Thesupernatant was used to estimate theextracellular lipase activity by using an olive oilemulsion as a substrate (olive oil 25%, 0.1 MNaOH 7.5%, polyvinyl alcohol (2%) 67.5%). • The free fattyacids released during the reaction were thentitrated with 0.05 M NaOH • One unit of lipaseactivity is defined as the amount of lipaseinducing the release of 1 mmol of fatty acid perminute at37℃and pH 7.

  8. Optimization of Fermentation Parameters Optimization of different nutrient and physical parameters for lipase production were studied by maintaining all factors constant except the one being studied. • pHvalues • Temperature • Selenium and Nickel • Urea and Ammonium nitrate

  9. Statistical Analysis The Pearson product momentcorrelation coefficient was calculated tomeasure the degree of linear relationshipbetween the variables. The correlation coefficient assumes a value between -1 and +1. If one variable tends to increase as the other decreases, the correlation coefficient is negative. Conversely, if the two variables tend to increase together the correlation coefficient is positive.

  10. Results And Discussions Growth conditions affect the synthesis of lipase by microorganisms. Carbon sources, nitrogen sources, the presence of activators and inhibitors, incubation temperature, pH, inoculum amount and oxygen tension can influence lipase production.

  11. Effect of temperature on lipase production by B.licheniformsKDP

  12. Lipase production was studied in the temperature range 25-60°C at an interval of 5°C. The maximum lipase activity (17.36 U/mL) was obtained at the temperature of 45°C .

  13. Effect of pH on lipase production by B.licheniformsKDP

  14. Maximum biomass and lipase production was obtained at an initial medium pH of 7.5 (18.69 U/mL).

  15. Effect of different concentration of Urea and Ammonium nitrate on lipase production by B.licheniformsKDP

  16. Themaximum lipase yield and biomass were found to be in medium containing 0.1mg/l urea and 1mg/l ammonium nitrate. Theseexperiment data indicates that the ammonia nitrate is the better nitrogen source than urea.

  17. Effect of different concentration of Se on lipase production by B.licheniformsKDP

  18. The maximum biomass and enzyme production (16.23 U/mL) were obtained in media supplemented with 60μM/L of Se

  19. Effect of different concentration of Ni on lipase production by B.licheniformsKDP

  20. The maximum lipase production was found at 80μM/L Ni grown culture. The perfect positive correlation (γ= +1) was found for 60-80μM/L of Ni grown culture.

  21. Conclusions Ammonium nitrate was found to be a best nitrogen source than urea. The new isolate produced their maximum extracellular lipase at pH 7.5 (18.69 U/mL). Maximum extracellular (17.36 U/mL) lipase activity was obtained at 45°C. Licheniformswas a thermophilic organism and it could tolerate higher temperature. The metal ions Se and Ni supported maximum production of lipase at 60μM/L and 80μM/L respectively.

  22. END

More Related