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RNA: Structure & Function

RNA: Structure & Function. What is RNA?. RNA is a nucleic acid called Ribonucleic Acid Functions of RNA: RNA transfers genetic information from the nucleus (DNA) to the cell (proteins) for use. Makes up ribosomes Helps assemble proteins.

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RNA: Structure & Function

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  1. RNA:Structure & Function

  2. What is RNA? RNA is a nucleic acid called Ribonucleic Acid Functions of RNA: • RNA transfers genetic information from the nucleus (DNA) to the cell (proteins) for use. • Makes up ribosomes • Helps assemble proteins

  3. The Structure of RNA(Similar to DNA with several key differences) • Made up of a repeating strand of nucleotides, contains all 3 parts similar to DNA (sugar, phosphate, nitrogen base) • The sugar in RNA is called Ribose • Contains the nitrogen base Uracil instead of Thymine. Uracil will bind to Adenine (like thymine did) • RNA is single strand How does DNA compare to RNA?

  4. Types of RNA There are 3 types of RNA, each with a different job 1. Messenger RNA (mRNA) 2. Transfer RNA (tRNA) 3. Ribosomal RNA (rRNA) Each type of RNA has a different structure that is related to its function.

  5. Messenger RNA (mRNA) • mRNA- single strand that carries messages from the DNA to the cytosol, so that it can be used make proteins.

  6. Transfer RNA (tRNA) • tRNA- is a cloverleaf shaped single strand that matches the amino acid to the correct sequence of mRNA

  7. Ribosomal RNA (rRNA) • rRNA- is a single strand in globular form, rRNA binds with proteins to make up ribosomes which are then used to make the proteins

  8. Why do we need RNA • Our body needs to make proteins in order to carry out cell functions. The instructions on how to make proteins are found in our DNA. • Enzymes can not read our DNA so it must be converted into RNA which our enzymes can read. This process is called… Transcription!

  9. How do we get RNA from the DNA message? Transcription Transcription allows DNA to stay in the nucleus, where its protected, and can continue making usable copies for the cell to manipulate.

  10. Transcription is… The process of making RNA from the DNA strands in the nucleus Location: Takes place in the nucleus of a cell

  11. Transcription Steps Step 1: RNA polymerase (enzyme) binds to DNA at the promoter region (a DNA sequence that signals the start of the gene and marks the start of transcription.) Step 2: RNA polymerase breaks the hydrogen bonds, unwinding the DNA double helix. Step 3: RNA polymerase reads the DNA, building a new RNA strand by adding one nucleotide at a time until it reaches a termination signal. Step 4: RNA polymerase releases the mRNA and the DNA recoils. Step 5: The RNA strand moves out of the nucleus through a nuclear pore into the cytosol.

  12. Transcription

  13. Transcription Animation

  14. Transcription Animations • http://www.youtube.com/watch?v=vJSmZ3DsntU • http://www.youtube.com/watch?v=ztPkv7wc3yU&feature=related • www.ncc.gmu.edu/dna/mRNAanim.htm • www.stolaf.edu/people/giannini/flashanimat/molgenetics/transcription.swf

  15. Let’s Transcribe some DNA… • DNA strand: A A T C C G T A T T • RNA strand: U U A G G C A U A A one more time….. • DNA strand: T A G C C T T G A T • RNA strand: A U C G G A A C U A And what are those base pairing rules we follow for transcription?

  16. What’s the end result of Transcription? 1 strand of mRNA which is now taken out of the nucleus into the cytosol!!

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