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Continuous-Flow Column Studies of Reductive Dehalogenation with Two Different Enriched Cultures: Kinetics, Inhibition, a

Continuous-Flow Column Studies of Reductive Dehalogenation with Two Different Enriched Cultures: Kinetics, Inhibition, and Monitoring of Microbial Activity . Lewis Semprini and Mark Dolan, Oregon State University Alfred Spormann and Sebastian Behrens, Stanford University.

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Continuous-Flow Column Studies of Reductive Dehalogenation with Two Different Enriched Cultures: Kinetics, Inhibition, a

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  1. Continuous-Flow Column Studies of Reductive Dehalogenation with Two Different Enriched Cultures: Kinetics, Inhibition, and Monitoring of Microbial Activity Lewis Semprini and Mark Dolan, Oregon State University Alfred Spormann and Sebastian Behrens, Stanford University Columns - Continuous Flow • Research Objectives: • Evaluate the transformation of chlorinated ethenes in continuous-flow column studies with cultures that have been kinetically characterized. • Determine if the derived kinetic models and parameters accurately simulate the spatial transformations in the columns. • Apply molecular methods, such as FISH and Real-Time PCR to determine the spatial distribution of the cultures and quantify the dehalogenating biomass within the column. • Apply RNA-based methods to determine energetically based TCE and VC-dehalogenating activity temporally and spatially within the columns. RNA-Based Method FISH Method

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