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René Wijffels, Michel Eppink, Maria Barbosa @ ReneWijffels

AlgaePARC biorefinery. René Wijffels, Michel Eppink, Maria Barbosa @ ReneWijffels. Contents. Technical and economical feasibility Process Photobioreactors Lipids Biorefinery Projects AlgaePARC AlgaePARC biorefinery SPLASH FUEL4ME MIRACLES. Can algae replace commodities of today?.

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René Wijffels, Michel Eppink, Maria Barbosa @ ReneWijffels

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  1. AlgaePARCbiorefinery René Wijffels, Michel Eppink, Maria Barbosa @ReneWijffels

  2. Contents • Technical and economical feasibility • Process • Photobioreactors • Lipids • Biorefinery • Projects • AlgaePARC • AlgaePARC biorefinery • SPLASH • FUEL4ME • MIRACLES

  3. Can algae replace commodities of today? • European market: • Fossil fuels: 400 Mtonnes/year • Polymers and chemicals: 100 Mtonnes/year • Palm oil: 7 Mtonnes/year • Soy: 8 Mtonnes/year • Global microalgae market today: • < 10,000 tonnes/year

  4. Develop this process is a sustainable and economical way within the next 10 -15 years To develop a more sustainable and economically feasible process, all biomass components should be used

  5. From basic toapplied research • Resources • Efficientuse of sunlight • Reduction of energy input • Use of residualnutrients • Strains • Lipidaccumulation • Metabolic Engineering • Implementation • Scale-up • Biorefinery • Design scenarios/LCA’s

  6. Photobioreactor design

  7. Antennasizemutants • Wild type cells • Cellsoversaturated: heat dissipation • Antennasizemutants • Light-saturation at higher light intensity • Less energy wasted. Tim de Mooij Turbidostat

  8. Behaviourduringday/nightcycles Lenneke de Winter • The aim of this research is toobtain more knowledge in the circadianbehaviour of the cellcycle of lipidproducingmicroalgaegrown in photobioreactors. Thiswillprovidewaystooptimize the currentproductionprocessunder outdoor day/nightcycles. • Neochlorisoleoabundans is grown in a lab-scalephotobioreactorundersimulated outdoor conditions. • The circadianrhythm of the cellcycle is analysedusing flow cytometry. • In addition, cellcomposition (lipids, starch, proteins) during the cellcycle is investigated.

  9. Lipids e e e e e

  10. Lipid accumulation N- N+ Breuer G., Lamers P.P. , Martens D.E. , Draaisma R.B., Wijffels R.H. (2012) The impact of nitrogen starvation on the dynamics of triacylglycerol accumulation in nine microalgae strains. Bioresource Technology 124: 217-226

  11. Genetic engineering Stefan Aanstoot • Lipids • Strain improvement towards a desired fatty acid profile • Strainimprovementtowards a increased TAG production • Hydrocarbons • Botryococcus • InsertBotryococcusgenesforhydrocarbons in Chlamydomonas • Mutagenesis and direct gene targeting approaches • Transcriptomics toobtaininsight in regulation Lenny de Jaeger

  12. TAG production: processoptimization Breuer et al. 2013

  13. Continuous TAG production Different light:Nratios Anne Klok NO3 N- medium Klok et al. 2013

  14. Richard PostmaProteinsfrom Green Sources • Development of a mild, continuous, scalabledisruptionandproteinextractiontechnologywithminimal energy requirements Soluble Insoluble Disruption Bead-milling (referencemethod) Enzymatic Pulsed Electronic Field Extraction Ionic liquid Polymerprecipitation Ultrafiltration

  15. AlgaePARC • Basic research is not sufficient • Gap between basic research and commercial applications • Continuous interaction between basic research and pilots • Follow up in demonstrations • Products • Scale • Production chain

  16. AlgaePARC • International centre of applied research • Intermediate between basic research and applications • Development of competitive technology (economics, sustainability) • Acquire information for full scale plants • Algal biomass for food, feed, chemicals and fuels

  17. Scenario: Lipids + Biogas energy Scenedesmus: €600/T Cost of production: €500/T

  18. Scenario: Lipid & protein biorefinery Nannochloropsis: €8,000/T Cost of production: €500/T

  19. AlgaePARC biorefinery • Fractionateallcomponents • Maintaintheirfunctionality • At low energy input

  20. EU-FP7 projects • INTESUSAL: demonstration project Portugal • ALGADISK: biofilmreactor; pilot Spain • FUEL4ME: biofuel project with pilots in Israël, Italy, Spain and Netherlands • SPLASH: biomaterials project with pilots in Spain and Netherlands • MIRACLES: biorefinery project for food and feed applicationswith pilots in Norway, Chile, Netherlands and Spain

  21. Education • Primary school • High schools • Design challengeand practical kit • BSc • Physiologyandprocess design • MSc • Bioreactor design • PhD • Designing of microalgae-basedproductionprocesses, for research and commercial purposes • Next edition: June 2014

  22. Algae for commodities • Demonstrate that biobased products have the quality to replace existing products • Convince we can produce economically in the future • Imagine we can reach the right market volumes • Create awareness: it is not going to be easy • Join forces • Create a long term commitment

  23. www.AlgaePARC.com Rene.Wijffels@wur.nl @ReneWijffels

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