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PHARMACOLOGY 545 Cardiovascular Pharmacology Cardiac SL ion transport systems Part II

PHARMACOLOGY 545 Cardiovascular Pharmacology Cardiac SL ion transport systems Part II 7 February 2005. Outline Physiological functions of cardiac SL Excitation-contraction coupling Regulation of pH i Metabolism Regulatory receptors Structure of cardiac sarcolemma

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PHARMACOLOGY 545 Cardiovascular Pharmacology Cardiac SL ion transport systems Part II

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  1. PHARMACOLOGY 545 Cardiovascular Pharmacology Cardiac SL ion transport systems Part II 7 February 2005

  2. Outline Physiological functions of cardiac SL Excitation-contraction coupling Regulation of pHi Metabolism Regulatory receptors Structure of cardiac sarcolemma Excitation-contraction coupling Regulation of pHi

  3. Na+-Ca2+ exchanger(NCX)

  4. NCX 3Na+ Ca2+ ENCX= 3ENa-2ECa

  5. E-C coupling in mammalian heart

  6. measuring NCX activity • transcribe cDNA into cRNA in vitro • inject cRNA into Xenopus oocytes NCX cRNA NCX cRNA NCX

  7. excised giant patch clamp extracellular side Ca2+ (8 mM) NCX Na (100 mM) 1 mM cytoplasmic side

  8. Ca2+ dependence of NCX

  9. NCX 3Na+ Ca2+ ENCX= 3ENa-2ECa

  10. E-C coupling in mammalian heart

  11. Store-operated channels(SOC)

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