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DNA / RNA

DNA / RNA. Taylor Stevenson Feb 1 st , 2011. Outline. Canonical Genetic Properties Chemical Structures Physical Structures ds DNA ss RNA Applications. Central Dogma. DNA. RNA. Protein. Primary Structure of DNA / RNA. Negatively Charged Directional Stable. OH. OH. OH. OH.

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DNA / RNA

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  1. DNA/RNA Taylor Stevenson Feb 1st, 2011

  2. Outline • Canonical Genetic Properties • Chemical Structures • Physical Structures • dsDNA • ssRNA • Applications

  3. Central Dogma DNA RNA Protein

  4. Primary Structure of DNA/RNA • Negatively Charged • Directional • Stable OH OH OH OH Modified from http://en.wikipedia.org/wiki/File:DNA_chemical_structure.svg

  5. Secondary Structure of DNA/RNA • H-bonding • A(T/U) , GC • Reversibility Modified from http://en.wikipedia.org/wiki/File:DNA_chemical_structure.svg

  6. Tertiary Structure of dsDNA • 10.5 bp/turn • 3.6 nm/turn • 0.34 nm/bp • 2.0 nm wide Modified from Lehninger et.al. Principals of Biochemistry 4th Ed

  7. Tertiary Structure of dsDNA • 10.5 bp/turn • 3.6 nm/turn • 0.34 nm/bp • 2.0 nm wide Modified from Lehninger et.al. Principals of Biochemistry 4th Ed

  8. Varying Structure of dsDNA • A is dehydrated. • B is “normal”. • Z is CGCGC... Modified from Lehninger et.al. Principals of Biochemistry 4th Ed

  9. Tertiary Structure of ssRNA • mRNA • tRNA Modified from http://upload.wikimedia.org/wikipedia/commons/b/ba/TRNA-Phe_yeast_1ehz.png Modified from http://upload.wikimedia.org/wikipedia/commons/4/4a/Pre-mRNA-1ysv.png-tubes.png

  10. Uses of DNA/RNA as a Generic Material

  11. dsDNA as a building block • Genetic Tools for Generic Manipulation: • PCR • Digestion • Annealing • Ligation Modified from Luo et.al. Nature Letters 2006 5.

  12. DNA/RNA as a biosensor ss-aptamer fluorescent tag Modified from http://aptamer.icmb.utexas.edu/images/aptamer-rre_rev.jpg Modified from Benvin et. al. J. Am. Chem. Soc. 2007 7.

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