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出力チーム

出力チーム. なぜ素早い出力が必要？. レスポンステスト. レスポンステスト. 液体培地. 固体培地. Under Construction. Response Test. Pick. Measure. Measure. Glucose 0.2%. Glucose 0.2%. IPTG 0.1mM (X-Gal 20μg/mL). コロニーリフト. IPTG 0.1mM (X-Gal 20μg/mL). テスト結果. Note:LacZ. LacZ-Xgal の出力までが遅いのは Xgal 濃度が低いため？

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出力チーム

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  1. 出力チーム

  2. なぜ素早い出力が必要？

  3. レスポンステスト

  4. レスポンステスト 液体培地 固体培地 Under Construction

  5. Response Test Pick Measure Measure Glucose 0.2% Glucose 0.2% IPTG 0.1mM (X-Gal 20μg/mL) コロニーリフト IPTG 0.1mM (X-Gal 20μg/mL)

  6. テスト結果

  7. Note:LacZ • LacZ-Xgalの出力までが遅いのはXgal濃度が低いため？ • Xgal 20μg/mLを40μg/mL,80μg/mLにしてレスポンスタイム計測

  8. Note:LacZ • Miller assay: LacZ活性の定量化 ONPG:Ortho-nitrophenyl-β-D-galactopyranoside Ortho-nitrophenolの吸光度を測定しLacZ活性を調べる

  9. Note:Luciferase • Luciferaseの反応：Coelenterazineの酸化 • Coelenterazine:膜透過性があるHydroxypropyl-β-Cyclodextrinを加えると水中での安定性が向上 Coelenterazine β-Cyclodextrin

  10. 来週の予定 • 他の出力を試し、評価する(Luciferase, mCherry)

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