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Third generation sequencing & Comparison of NGS Technology Platforms

Third generation sequencing & Comparison of NGS Technology Platforms. Sravanthi Joginipelli. Available NGS technology platforms. Three commercially available NGS platforms, i.e. Second generation sequencing technologies 1. Roche (454) FLX Genome Sequencer, 2. Illumina Genome Analyzer II

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Third generation sequencing & Comparison of NGS Technology Platforms

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  1. Third generation sequencing & Comparison of NGS Technology Platforms Sravanthi Joginipelli

  2. Available NGS technology platforms Three commercially available NGS platforms, i.e. Second generation sequencing technologies 1. Roche (454) FLX Genome Sequencer, 2. Illumina Genome Analyzer II 3. Applied Biosystems’ SOLiDTM (Sequencing by Oligo Ligation and Detection) Third generation sequencing platforms • Helicos • HeliScope • Pacific Biosciences

  3. Third generation sequencing 3rd generation PacBio RS • Single Molecule Sequencing Technology (tSMS) • No amplification • 10Gb os sequence data per 8 day run • Single Molecule Real Time (SMRT) sequencing technology (PacBio RS)

  4. Roche & illumina analyzers Genome Sequencer FLX Illumina Genome Analyzer

  5. Comparison Roche and Illumina

  6. Roche, Illumina and AB SoLiD

  7. Thank you

  8. References • Dames S, Durtschi J, Geiersbach K, Stephens J, Voelkerding KV, Comparison of the Illumina Genome Analyzer and Roche 454 GS FLX for resequencing of hypertrophic cardiomyopathy-associated genes. • DNA Microarray Inc, “Genomic Services” http://www.dnamicroarray.com/services_genomics_ngs.html • Pacific Biosciences, http://www.pacificbiosciences.com/ • 454 lifesciences http://www.roche-applied-science.com/

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