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Resistance – Thermal and Other

Resistance – Thermal and Other . Robert L. Buchanan DHHS Food and Drug Administration Center for Food Safety and Applied Nutrition. Thermal Resistance of E. sakazakii – Laboratory Trials. Nazarowec-White and Farber (1997) Open stainless steel tubes in constant temperature water bath

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Resistance – Thermal and Other

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  1. Resistance – Thermal and Other Robert L. Buchanan DHHS Food and Drug Administration Center for Food Safety and Applied Nutrition

  2. Thermal Resistance of E. sakazakii – Laboratory Trials • Nazarowec-White and Farber (1997) • Open stainless steel tubes in constant temperature water bath • 5 pooled food strains and 5 pooled clinical strains • Edelson-Mammel and Buchanan (2003) • Submerged coil apparatus • 12 individual strains – food and clinical

  3. Thermal Resistance • D-Value: • Time at a given temperature needed to reduce a microbial population by 90% • Z-Value: • Change in temperature needed to change D-value by 90%

  4. Thermal Death Time Curves for 2 Enterobacter sakazakii Strains Heated at 58C D = 591.9 sec D = 30.5 sec

  5. Distribution of D58°C-values for 12 Enterobacter sakazakii strains

  6. Comparison of D58°C-Values for Different Enterobacteriaceae

  7. Effect of Heating Temperature on D-Value for E. sakazakii 607

  8. Reported Z-values • Nazarowec-White and Farber (1997) • Pooled food isolates: 5.6°C • Pooled clinical isolates: 6.0 °C • All: 5.8 °C • Edelson-Mammel and Buchanan (2003) • Strain 607: 5.6 °C

  9. Predicted Inactivation • Results of these heating trials indicate that heating rehydrated infant formula at 70°C for even a few seconds will result in a substantial inactivation of E. sakazakii

  10. Effect of Temperature of Water Used to Rehydrate Infant Formula • Inoculated dried infant formula in baby bottle • Add water pre-heated to different temperatures • Cap and agitate periodically for 10 minutes • Analyze rehydrated formula for E. sakazakii

  11. Temperature Decline During Rehydration of Infant Formula

  12. Rehydration of Dried Infant Formula Lower Limit of Detection

  13. Impact of Heating on Nutrient Content • Conducted by Atlanta Center for Nutrient Analysis (ACNA) • Used boiling water as the worst case and compared against control • Analyses done in triplicate • Results expressed in terms of units/100 cal

  14. Impact of Heating on Nutrient Content

  15. Impact of Heating on Nutrient Content

  16. Impact of Heating on Nutrient Content

  17. Thermal Resistance of E. sakazakii – Pilot Plant • Nazarowec-White et al. (1999) • Pooled strains? • HTST pasteurizer

  18. Effectiveness of Pasteurization

  19. Resistance to Other Treatments • Great deal of information available about Enterobacteriaceae in general

  20. Resistances If Like Other Enterobacteriaceae • Not heat resistant • Moderate acid resistance if adapted • Moderate alkali resistance if adapted • Low to moderate chlorine resistance • Low to moderate irradiation resistance • Will remain viable in refrigerated and frozen products for extended periods, particularly if neutral pH • Moderate to good resistance to drying

  21. Resistance to Other Treatments • Little published information available specifically about E. sakazakii • Even less available on distribution of resistances

  22. Other Resistances • Isolation from dried foods indicates resistance to drying • Isolation from seeds treated with hypochlorite suggest that at least some strains may be relatively resistant to chlorine (Okuda et al., 1994) • Relatively sensitive to the growth inhibiting effects of chitosans (No et al., 2002)

  23. Resistance to Dehydration

  24. Summary • Not a particularly thermally resistant microorganism • Substantial diversity in thermal resistance among strains • Good agreement among studies • Inactivation at temperatures above 70°C, even for a few seconds • Specific information on its resistance to other treatments is generally lacking

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