1 / 10

See why.

See why. Massive Analysis of cDNA Ends: MACE. How it works. cDNA population bound to Streptavidine. AAAAAAA-3’ TTTTTTT-5’. 5’ 3’. cDNA. Streptavidin-Beads. 5’ 3’. AAAAAAA-3’ TTTTTTT-5’. cDNA. 5’ 3’. AAAAAAA-3’ TTTTTTT-5’. cDNA. AAAAAAA-3’ TTTTTTT-5’. 5’ 3’.

addison
Télécharger la présentation

See why.

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. See why.

  2. Massive Analysis of cDNA Ends: MACE How it works cDNA population bound to Streptavidine AAAAAAA-3’ TTTTTTT-5’ 5’ 3’ cDNA Streptavidin-Beads 5’ 3’ AAAAAAA-3’ TTTTTTT-5’ cDNA 5’ 3’ AAAAAAA-3’ TTTTTTT-5’ cDNA AAAAAAA-3’ TTTTTTT-5’ 5’ 3’ cDNA AAAAAAA-3’ TTTTTTT-5’ 5’ 3’ cDNA AAAAAAA-3’ TTTTTTT-5’ 5’ 3’ cDNA

  3. Massive Analysis of cDNA Ends: MACE How it works Fragmentation AAAAAAA-3’ TTTTTTT-5’ Streptavidin-Beads AAAAAAA-3’ TTTTTTT-5’ AAAAAAA-3’ TTTTTTT-5’ AAAAAAA-3’ TTTTTTT-5’ AAAAAAA-3’ TTTTTTT-5’ AAAAAAA-3’ TTTTTTT-5’ 100-200 bp

  4. Massive Analysis of cDNA Ends: MACE How it works 2nd generation sequencing of 50-100 bp AAAAAAA-3’ TTTTTTT-5’ AAAAAAA-3’ TTTTTTT-5’ AAAAAAA-3’ TTTTTTT-5’ AAAAAAA-3’ TTTTTTT-5’ AAAAAAA-3’ TTTTTTT-5’ AAAAAAA-3’ TTTTTTT-5’

  5. Massive Analysis of cDNA Ends: MACE How it works 2nd generation sequencing of 50-100 bp AAAAAAA-3’ TTTTTTT-5’ AAAAAAA-3’ TTTTTTT-5’ AAAAAAA-3’ TTTTTTT-5’ AAAAAAA-3’ TTTTTTT-5’ AAAAAAA-3’ TTTTTTT-5’ AAAAAAA-3’ TTTTTTT-5’

  6. Massive Analysis of cDNA Ends: MACE How it works Counting, mapping, assembly, SNP detection 1 1 4

  7. MACE AT CG AT AAAAAAAA TTTTTTTT cDNA Reads SNPs withenoughcoverage: 2 RNA-Seq AT CG AT AAAAAAAA TTTTTTTT cDNA SNPs withenoughcoverage: 0 Reads

  8. Massive Analysis of cDNA ends: MACE SNP-Analysis SNP Rattus norvegicus, laboratory strain, ClustalX view of alpha actin MACE tags

  9. High resolution: rare transcripts included! Transcript-species copy-numbers and distribution MACE Microarray

  10. Massive Analysis of cDNA Ends: MACE Advantages • High-resolution: • only one specific tag is sequenced per transcript • even low-level expressed transcripts are analyzed • High SNP-coverage: • only the highly polymorphic 3’ ends are sequenced • high coverage for reliable SNP detection is warranted • High throughput & low costs: • hundreds of samples can be analyzed simultaneously • multiplexed sequencing reduces costs while still providing high resolution

More Related