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Arterieel endothelium

Fibreus weefsel. Vasculaire gladde spier. Arterieel endothelium. MAPCellen and vasculaire ziektes. Arterieel endothelium? Vasculaire gladde spier? Bioartificiele arterie? In vivo therapie voor bvb. gangreen?. TGF- b 1 +/- PDGF-BB induces functional (v)SMCs from MAPCellen. I (pA/pF).

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Arterieel endothelium

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  1. Fibreus weefsel Vasculaire gladde spier Arterieel endothelium

  2. MAPCellen and vasculaire ziektes Arterieel endothelium? Vasculaire gladde spier? Bioartificiele arterie? In vivo therapie voor bvb. gangreen?

  3. TGF-b1 +/- PDGF-BB induces functional (v)SMCs from MAPCellen I (pA/pF) RAOSMC rMAPC I (pA/pF) Passage 1 Day 0 Day 2 Day 6 SM22 SM22a Pass 1 TGF-b1 + PDGF-BB SMaActin I (pA/pF) F-rMAPC Day 6 Pass 1 SMaActin RAOSMC SM22 IgG Specific antibody JEFF ROSS, J Clin Invest 2006

  4. MAPCellen and vaatziektes Arterieel endothelium: JA Vasculaire gladde spier: JA Bioartificiele arterie? In vivo therapie voor bvb. gangreen?

  5. Week 5 Rat Aorta MAPCel-gladde spier kan “contractiele” tube vormen Week 5, no mech. cond. MAPC-SMCs + fibrin gel into tubular cavity Cells are entrapped in “gel” formed around a nonadhesive mandrel Cell-induced gel contraction around mandrel CollagenNucleiFibrin JEFF ROSS, JCI 2006

  6. Conditioneren van de tube om contractiliteit te verhogen MAPC-SMC ring constructs are subjected to cyclic distention for 3 weeks at 5% strain, 0.5 Hz, and 12.5% duty cycle JEFF ROSS, JCI 2006

  7. ET-1 ET-1 10 mg 5 min 30 mg 5 min MAPC-SMC Mechanical MAPC-SMC Static MAPCel-gladde spier tube kan samentrekken en relaxeren zoals arterieel bloedvat Traces of MAPC-SMC rings 60 mM KCl 60 mM KCl 50 mg Rho-Kinase inhibitor relaxation 5 min 10 mM ACh 60 mM KCl 10 mM Y-27632 MAPC-SMC Static MAPC-SMC Mechanical Rho-Kinase Inhibitor MAPC-SMC Mechanical Vasodilation JEFF ROSS, JCI 2006

  8. Arterie? • Moet kunnen druk van ~1,000mmHg weerstaan; Momenteel ~300mmHg • Hoe creeren we endothelium aan binnenzijde? • Heel waarschijnlijk niet goed gehecht, en laat loss door “sheer” stress van bloed • Moeten ervoor zorgen dat er geen klonters ontstaan

  9. MAPCellen and vasculaire ziektes Arterieel endothelium: JA Vasculaire gladde spier: JA Bioartificiele arterie? NOG NIET In vivo therapie voor bvb. gangreen?

  10. Perifeer ischemie model Acute Ischemie: Femorale arterie geligeerd Onmiddelijke behandeling “Chronische” Ischemie; ~ klassieke perifere vasculaire insufficientie Iliacale arterie geligeerd Behandeling 5 dagen later extensieve celdood, fibrose en verlies van lidmaat Minimale celdood Luttun et al J Clin Invest In Press

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