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  1. Supplemental Figure 2. Little changes in the levels of p53 and p21 by CMZ. C6 glioma cells were untreated (lane 1) or treated with 20 µM CMZ (lane 2) or DMSO (lane 3) for 16 h before cell harvest. Equal amounts of the whole cell homogenates (30 µg protein/well of mini-gels) were separated on 12% SDS-polyacrylamide gels followed by immunoblot analysis using the specific antibody against p53 (top), p21 (middle), or actin (bottom). This result represents a typical result of two independent experiments.

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