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C. Difficile Testing Recommendations

C. Difficile Testing Recommendations. Alfred DeMaria, Jr., M.D. Medical Director Bureau of Infectious Disease    Prevention, Response and Services State Epidemiologist. Testing for C. difficile. Testing for CDI:

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C. Difficile Testing Recommendations

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  1. C. Difficile Testing Recommendations Alfred DeMaria, Jr., M.D. Medical Director Bureau of Infectious Disease    Prevention, Response and Services State Epidemiologist

  2. Testing for C. difficile • Testing for CDI: • Culture and toxin neutralization not practical in most laboratories (but gold standard) • EIA for toxins A and/or B is easy to do, but not very sensitive (giving false negatives) • GDH tells you C. difficile is present, but doesn’t tell you if it is toxin-producing • GDH+ plus EIA+ = toxigenic C. difficile, but still have sensitivity problem related to EIA • PCR more sensitive and as specific, and it identifies A&B toxin gene

  3. C. Difficile Test “Rules” • Only test unformed diarrheal stools • Do not do repeat testing • Do not test for “cure”

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