1 / 1

Immunostaining Analysis of HIF-1α and HIF-2α in Muscles Under Stretch and Prazosin Treatment

This supplementary figure (1) presents immunostaining results for HIF-1α (A-C) and HIF-2α (D-F) in skeletal muscle subjected to control (A,D), 7-day stretch (B,E), and 7-day prazosin treatment (C,F). Panels A-C feature DAPI counterstaining for nuclear visualization (blue), while panels D-F are counterstained with lectin to highlight capillaries (red). Representative images were captured at 400x magnification. Arrows indicate positive myonuclear staining for HIF proteins, and the arrowhead in Panel B highlights signaling within the pericapillary region.

akasma
Télécharger la présentation

Immunostaining Analysis of HIF-1α and HIF-2α in Muscles Under Stretch and Prazosin Treatment

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Control 7 day Stretch 7 day Prazosin A B C D E F Supplementary Figure 1: Immunostaining of HIF-1a (A-C) and HIF-2a (D-F) in control (A,D), 7 day stretch (B,E) or 7 day prazosin (C,F) -treated muscles. Panels A-C were counter-stained with DAPI to visualize nuclei (blue) and panels D-F were counterstained with lectin to visualize capillaries (red). Representative images of each condition were obtained at a magnification of 400x. Arrows point to positive myonuclear staining for HIF-a proteins. Arrowhead in Panel B points to signal within the pericapillary area.

More Related