SINKRONISATION

1. SINKRONISATION SPECIFIK INSTRUCTIONAL PURPOSE STUDENT CAPABLE TO SYNCRONISATION ON LIVESTOCK

2. INTRODUCTION • Tecnology Function in Farm : Efficiency of Reproduction Conception Rate Calving Interval Puberty Age Service per Conception

3. Estrous Snap  Make shorter of estrous cycle on a cow Estrous Syncronisation  Make shorter of estrous cycle on a group of livestock

5. ESTROUS SYNCRONISATION • Make a group livestock estrous together  Artificial Insemination  gestation  partus of calv with the same age • Efficienci : - cycle shorter - cost of insemination - easy maintenance • For teraphy of disease

6. Analisys SWOT of technology estrous sincronisation • STRONG • WEAKNESS • OPPORTUNITY • THREAT

7. STRONG • IS CHEAP TECHNOLOGY • NEED COMPETENT PERSONAL • NEW OPPORTUNITY • EFEKTIF FOR ↑ OF REPRODUCTION EFFICIENCY • WEAKNESS • PRICE OF HORMONE IS EXPENSIVE • LOW OF GESTATION • LOW INFORMATION • HIGHT COST OF OPERATIONAL FOR LITTLE POPULATION

8. OPPORTUNITY • PERFECTION FOR TECHNOLOGY • NUMBER OF POPULATION ↑ • RERPODUCTION DISTURBANCE ↑ • THREAT • AI COST  EXPENSIVE CHANGE NATURAL MATTING • TRADITIONAL BEHAVIOUR

9. HORMONE APLICATION • PROGESTERONE • PROSTAGLANDHINE F2 PRINCIPLE ESTROUS SNAP WITH HORMONE PREPARAT BASED ON FEED BACK PRINCIPLE FROM REPRODUCTION HORMONE

10. PROGESTERONE • PRID (PROGESTIN RELEASE INTRAVAGINAL DEVICE) SPIRAL HUMAN • CIDR (CONTROL INTRA VAGINAL DEVICE RELEASE) • PRIVASIS (P4 INTRA VAGINAL SILICONS SPONS • IMPLANT CYLASTIC => SYNCROMED B KB SUSUK UNDER SKIN OF NECK / EAR • PRINCIPLE : AS CL SINTETIC IMPLANT IN VAGINA 9-14 DAYS • MIN DOSE 40 mg – 1 g AS DEPOPROVERA => CONTENT P4 • THE SURLUS : DIDN’T SEE ESTROUS PHASE

11. WEAKNESS : • DIDN’T COMFORTABLE • IF CONTRACTION => IT’S GO OUT • BETTER : IMPLANTATION UNDER SKIN OF EAR • IMPLANT IN THE NECK  MAYBE DISAPPEAR • IM ??? HOW STOPPED?? • SPECIALLY IN SOW  BY FOOD DURING 9 DAYS • GOOD ESTROUS  P4 COMBINE WITH ESTRADIOL WHY ???

12. HOW ABOUT MECHANISME • PROGESTERON APLICATION • PROSTAGLANDIN F2 APLICATION

13. PROSTAGLANDINE F2 • APLICATION IN LUTEAL PHASE • THE RULES : THERE IS CL & DIDN’T GRAVID • PG = AS LEMAK, LOW BM = 400 Da So Can Used : I.Ut, IM, I.Vul, I.Ovr, I.Vn (ABORTUS INDUKTION) • COMMERCE PREPARAT : • GLANDIN F, • LUTELYSE, • PROSOLVIN, • PROSTAVET

14. PG WITH 1 X APLICATION17/21 x 100 ekor = 75% In Order 100% with 2 X APLICATION Lutheal Phase Foliculer Phase 16 – 17 days 4-5 days 11 DAYS 75% 100% PG 1 EST PG2 EST 2-3 Hr 9-14 Hr 9 Days – 3 Days = 6 Days  EARLY OF LUTEAL PHASE 11 Days – 3 Days = 8 Days => in LUTEAL PHASE

15. DOSIS PROSTAGLANDIN F2

17. TUBA FALLOPII UTERUS KORPUS LUTEUM OVARIUM VENA UTERINA PG DARI ARTERI MERUSAK KL VENA ARTERI TRANSFER ARTERI OVARIKA MEMBELIT VENA

21. THANK YOU

22. SUPEROVULATION • ↑ COUNT OF OVULATION IN 1 NORMAL ESTROUS PERIODE • NEED MANIPULATION TO ↑ OVUM • SUPEROVULASI INDUCTION USED HORMONE PREPARAT • COMBINE FROM GONADOTROPHINE HORMONE

23. INDIVIDUAL RESPONSE INSUPEROVULATION PROGRAME, DEPENDED ON : • POTENTION &HORMONE DOSE HORMON YANG DIBERIKAN • COMPARISON OF GONADOTROPHINE • FRECUENCY OF INJECTION • SPECIES ANIMAL; FAMILY; AGE & FOOD

24. HORMONE APLICATION • COMBINATION OF HORMONE • FSH & LH • PMSG & hCG

25. PMSG (PREGNANT MARE SERUM GONADOTROPHINE) * MARE GESTATION = 40 -120 DAYS. WHY?....FOR WHAT?.... * < 40 DAYS OR >120 DAYS DIDN’T HAVE PMSG. WHY…? * IN GESTATION NEED P4  UNTIL 40 DAYS ENAUGH FROM CL GRAVIDITATUM

26. IN 40 DAYS NEED PMSG  FSH THE FOLICLE GROWTH BUT NOT OVULATION  LUTEINISATION CL ASCESORIS  PRODUCE P4 CL GRAVID + CL ASCESORIS  P4 ↑ > 120 DAYS  FUNCTION OF P4 FROM CL ASCESORIS CHANGED FROM PLACENTA UNTIL PARTURITION

27. hCG (human Chorionic Gonadotrophine) • hCG  LH • from : HUMAN URINE IN GRAVID TRIMESTER I • FUNCTION IN HUMAN : STIMULATION OF GONAD IN ORDER TO CL GRAVIDITATUM CONSIST TAKE CARE • IN SUPEROVULATION : HELP LH ENDOGEN FOR DISOSIASI FOLICLE WALL IN ORDER TO OVULATION

28. WHEN PMSG INJECTED?? • IF FSH LOWER IN BLOOD (LUTEAL PHASE) 9 – 14 DAYS OF ESTROUS CYCLEUS BIRAHI • IF FSH ↑  DIDN’T BENEFIT • IN ORDER TO KNOW  NEED ESTROUS SYNCRONISATION • ONLY 1 INJECTED

29. HOW ABOUT PMSG?? • LONG ACTION = 108 HOURS (5-6 DAYS) • ESTROGENIK EFECT  DISTURB OF • IMPLANTATION • DOSE 2000 – 3000 IU (FOLIGON) • FOR OVUM PRODUCE  DIDN’T NEED • MATTING • FOR ET  NEED MATTING • PHYSIOLOGIS CONDITION OF DONOR • MUST THE SAME OF RESIPIEN

30. SUPEROVULATION with PMSG & hCG – Sinkron with PG hCG IB Pagi PMSG DONOR PG1 11Day PG2 9Day 2Day PG3 3Day EST 7Day Flushing 1/6 12/6 21/6 23/6 26/6 3/7 IB Sore RESIPIEN PG1 11 Day PG2 3Day EST 7 Day Transfer 12/6 21/6 23/6 26/6 3/7 DOSE of PMSG : 2000 – 3000 IU DOSE of hCG : 1500 -2000 IU

31. SUPEROVULATION (PMSG-hCG) - Sinkron (P4) OUT PMSG hCG IB Pagi DONOR PRID 9 Day 3 Day EST 7 Day Flushing 1/6 10/6 13/6 20/6 IB Sore OUT RESIPIEN PRID 9 Day 3 Day EST 7 Day Transfer 1/6 10/6 13/6 20/6 KOMBINASI PG DOSIS PMSG : 2000 – 3000 IU DOSIS Hcg : 1500 -2000 IU DOSIS P4 DALAM PRID = 40 mg – 1 g BIRAHI LEBIH BAGUS

32. WHEN FSH INJECTED?? • BOOSTER INJECTED (4 DAYS: MORNING & EVENING) • EVERY 12 HOURS • COUNT 40 mg • WITH DOSE DECREASE : 6, 5, 4 , 3 mg • IN ORDER TO KNOW  NEED ESTROUS SYNCRONISATION • MASA KERJA PENDEK • S/ TDK BERSIFAT ESTROGENIK • S/ PEROLEHAN EMBRIO DG KWALITAS LEBIH BAIK • U/ PROD. OVUM TDK PERLU DIKAWINKAN • U/ ET PERLU DIKAWINKAN • SYARAT KONDISI FISIOLOGIS DONOR=RESIPIEN

33. HOW ABOUT FSH?? • SHORT ACTION = 12 HOURS • DIDN’T ESTROGENIK EFECT • KWALITAS EMBRYO IS BETTER GOOD • FOR OVUM PRODUCE  DIDN’T NEED • MATTING • FOR ET  NEED MATTING • PHYSIOLOGIS CONDITION OF DONOR • MUST THE SAME OF RESIPIEN

34. SUPEROVULATION WITH FSH & LH FSH PAGI FSH PAGI FSH PAGI LH IB Pagi FSH PAGI DONOR PG1 11Day PG2 9 Day PG3 3Day EST 7Day Flushing 1/6 12/6 21/6 22/6 23/6 24/6 27/6 4/7 FSH SORE FSH SORE IB Sore FSH SORE FSH SORE RESIPIEN PG1 11 Day PG2 3Day EST 7Day Transfer 12/6 23/6 27/6 4/7 FSH I : 6 mg FSH II : 5 mg FSH III : 4 mg FSH IV : 3 MG TOTAL 40 mg

35. FINISH & THANK YOU