Measurement of Laccase Activity in Transplastomic Lines

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This study details the measurement of laccase enzyme activity in transplastomic lines using leaves with 30 µg of total soluble protein. The assay was conducted in a 20 mM sodium acetate buffer at pH 5.0. Laccase activity was assessed by spectrophotometry at 420 nm with 2 mg/ml of ABTS (2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate)). The results are presented as mean ± SD with n=3. The analysis includes vector control (vc) and two laccase-expressing lines (L-2 and L-6).

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Jan 21, 2026

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Measurement of Laccase Activity in Transplastomic Lines

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S-figure 1 S-Fig. 1 Measurement of laccase activity in transplastomic lines. 30 µg total soluble protein from leave in 20 mM sodium acetate buffer (pH 5.0) was used. Laccase activity was determined using a spectrophotometer at 420 nm with 2 mg/ml of 2,2.-azinobis-(3-ethylbenzthiazoline-6-sulphonate). Data are expressed as mean ± SD (n=3). vc, vector control; L-2 and L-6, laccase-expressing line 2 and 6.