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Reconstructing 3D Objects from Microscopic 2D Images: Crosswise and Lengthwise Techniques

This lab session focuses on the principles of reconstructing three-dimensional (3D) objects from two-dimensional (2D) microscopic images. Students will explore various slicing techniques, including crosswise and lengthwise slices, to create accurate 3D representations of biological structures. The session includes hands-on activities utilizing plant slides and human tissue samples to understand how different sections influence the reconstruction process. By the end, participants will be proficient in distinguishing between cross sections and long sections, enhancing their understanding of microscopy.

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Reconstructing 3D Objects from Microscopic 2D Images: Crosswise and Lengthwise Techniques

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  1. 3-D Sections

  2. Lab 25 – Thinking About Microscopic Images

  3. Reconstructing 3D objects from 2D images Crosswise slice: Lengthwise slice: Whole object: Crosswise slice Lengthwise slice: Whole object:

  4. Reconstructing 3D objects from 2D images Crosswise slices in a series through the object: Whole object:

  5. Reconstructing 3D objects from 2D images Crosswise slices in a series through the object: Whole object:

  6. Reconstructing 3D objects from 2D images Long Section Cross Section

  7. Reconstructing 3D objects from 2D images Crosswise slice: Diagonal slice: Whole object:

  8. Cross section or Long section?

  9. Plant Slidesc.s. (cross section) or l.s. (long section)

  10. Human Tissue/Organ c.s. (cross section) or l.s. (long section)

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