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Purification of viruses

E. &. O. O. &. Purification of viruses. Purification. To obtain the viral particles in a single form out of cell debris i.e. removal of all impurities. Purification outline. Preparation of harvest. Methods of purification. Criteria of purity. Maintenance of purified virus.

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Purification of viruses

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  1. E & O O &

  2. Purification of viruses

  3. Purification To obtain the viral particles in a single form out of cell debris i.e. removal of all impurities.

  4. Purification outline • Preparation of harvest. • Methods of purification. • Criteria of purity. • Maintenance of purified virus.

  5. Preparation of harvest Bacteria Insect Animal Plant

  6. Methods of purification • Filtration. • Centrifugation. • Adsorption. • Precipitation. • Solvent extraction. • Other methods.

  7. Methods of purification • Filtration. • Centrifugation. • Adsorption. • Precipitation. • Solvent extraction. • Other methods.

  8. Filtration : 1.Ultrafiltration 2. Gel filtration

  9. Ultra-filtration

  10. Gel filtration

  11. Methods of purification • Filtration. • Centrifugation. • Adsorption. • Precipitation. • Solvent extraction. • Other methods.

  12. centrifugation Differential Cushion Isopycnic Gradient

  13. Methods of purification • Filtration. • Centrifugation. • Adsorption. • Precipitation. • Solvent extraction. • Other methods.

  14. Adsorption : 1.Ion exchange 2. Heamadsroption

  15. Ion exchange chromatography

  16. Heamadsorption

  17. Methods of purification • Filtration. • Centrifugation. • Adsorption. • Precipitation. • Solvent extraction. • Other methods.

  18. Precipitation : 1. Chemical • Ammoniumsufate • PEG • Zinc acetae 2. Physical • Isoelectric piont (pH change)

  19. Methods of purification • Filtration. • Centrifugation. • Adsorption. • Precipitation. • Solvent extraction. • Other methods.

  20. Methods of purification • Filtration. • Centrifugation. • Adsorption. • Precipitation. • Solvent extraction. • Other methods.

  21. Other methods • Electrophoresis. • Serological methods. • Enzymatic reactions. • HPLC

  22. Electrophoresis

  23. HPLC

  24. Criteria of purity Chemical purity Physical purity Biological purity

  25. Criteria of purity E.M. Spectrophotometer serological Isoradiation Infectivity

  26. Criteria of purity E.M. Spectrophotometer serological Isoradiation Infectivity

  27. Marking

  28. Criteria of purity E.M. Spectrophotometer serological Isoradiation Infectivity

  29. Infectivity: 1. Local lesion assay 2. Plaque assay

  30. Virus preparation

  31. Criteria of purity E.M. Spectrophotometer serological Isoradiation Infectivity

  32. Criteria of purity E.M. Spectrophotometer serological Isoradiation Infectivity

  33. 4- Maintenance: The half life of infectivity can be measured in seconds at 60 oC; minutes at 37 oC, hours at 20oC , days at 4 oC and years at -70 oC or lower,(Fenner et al, 1987) Lyophlization

  34. Thanks

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