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PC12 α 2 cells

Project workflow. Behavioral tests. Immunohistochemical analysis of neurogenesis. Chronic mild stress (uCMS). Animal sacrifice. 1 st Team Workpackage #1. Dissection of brain regions. 3 wks. Pharmacological treatments 4 wks. BrdU. Monoamines determination. Plasticity related

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PC12 α 2 cells

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  1. Project workflow Behavioral tests Immunohistochemical analysis of neurogenesis Chronic mild stress (uCMS) Animal sacrifice 1st Team Workpackage #1 Dissection of brain regions 3 wks Pharmacological treatments 4 wks BrdU Monoamines determination Plasticity related gene expression( qRT-PCR ) Immunohistochemical analysis of neuroprotection/apoptosis with in situ hybridization Hippocampus, amygdala, prefrontal cortex , anterior cingulate cortex , hypothalamus, parietal, temporal lobes 2nd Team Workpackage #2 Analysis of apoptosis with in situ hybridization Knock-down or overexpression of β-arrestins 3rdTeam Workpackage #3 PC12α2cells Analysis of neuronal differentiation, apoptosis and signal transduction Hippocampus, amygdala, prefrontal cortex , acc , hypothalamus Plasticity related gene expression (Western blotting) Dominant negative mutant or overexpression ofNrf2 & CREB

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