1 / 35

Mycology – Introduction

Mycology – Introduction. Student Lab Division of Medical Technology Carol Larson MSEd, MT(ASCP). Mycoses. Superficial Subcutaneous Systemic Opportunistic. Characteristics of fungi. Eukaryotic Growth requirements Forms Mold Yeast. Hyphae. Septate. Aseptate. Hyphae. Hyaline.

amos-young
Télécharger la présentation

Mycology – Introduction

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Mycology –Introduction Student Lab Division of Medical Technology Carol Larson MSEd, MT(ASCP)

  2. Mycoses • Superficial • Subcutaneous • Systemic • Opportunistic

  3. Characteristics of fungi • Eukaryotic • Growth requirements • Forms • Mold • Yeast

  4. Hyphae • Septate • Aseptate

  5. Hyphae • Hyaline • Dematiaceous

  6. Mycelium • Mass of branching intertwined hyphae • Vegetative • Aerial • Fertile

  7. Vegetative types • Favic chandeliers • Nodular organs • Racquet hyphae • Spiral hyphae

  8. Reproduction • Identify fungi by: • Morphology of reproductive structures • Spores from vegetative mycelium or aerial fruiting bodies

  9. Asexual Reproduction • Conidia • Conidiophore • Arthroconidia

  10. Asexual Reproduction • Blastoconidia • Pseudohyphae • Chlamydoconidia • Chlamydospores

  11. Asexual Reproduction • Macroconidia • Microconidia • Phialoconidia • Phialide

  12. Asexual Reproduction • Annelloconidia • Annellide • Sporangiospores • Sporangium • Sporangiophore

  13. Sexual Reproduction • Perfect Fungi – has a sexual stage • Fungi Imperfecti – no know sexual stage • Spores

  14. Sexual Reproduction • Ascospores • Ascus • Ascocarp • Basidiospores • Zygospores

  15. In review … • Mycoses – fungal diseases • Characteristics of fungi • Growth requirements • Forms (mold, yeast) • Structures • Reproduction • Asexual • Sexual

  16. Fungal Culture Process • Specimen collection and transportation • Direct examination of specimen • Selection and inoculation of media • Evaluation of fungal growth • Serological testing • Antifungal susceptibility testing

  17. Specimen Collection • Specimen types • Collect from area most likely infected • Use sterile technique • Keep specimen moist • Label container properly • Transport right away • Process right away

  18. Direct Examination • Provides preliminary report • Guides MD in treatment of patient • Observe yeast phase of dimorphic • Gives clues to id causative agent • Inoculate special media • May require more than one direct examination method

  19. Direct Examination • Saline wet mount • Lactophenol cotton blue wet mount • 10% KOH preparation • Gram stain • Acid fast stain • India ink stain

  20. Direct Examination • Calcofluor white stain • Wright’s stain • Gomori Methenamine Silver stain • Periodic Acid Schiff stain

  21. Specimen Processing • Safety • Tube media preferred over plate media • Work in safety hood • Wear gloves and lab coat • Autoclave specimens and media • Disinfect work area daily

  22. Specimen Processing • Primary isolation media • Goal: isolate potential pathogens • Use non-selective and selective media • Proper ingredients • Incubation temperature • Incubation time • Incubation atmosphere

  23. Non-selective Media • Sabouraud dextrose agar • Brain heart infusion (BHI) with/without 5% blood and 1% glucose

  24. Selective Media • Mycosel agar • Inhibitory mold agar • Dermatophyte test medium

  25. Subculture / Identification Media • Neutral Sabouraud dextrose agar (Emmon’s) • Cornmeal-Tween 80 agar • Niger seed agar (Birdseed agar) • Tween 80 / Oxgall / caffeic acid agar • Potato dextrose agar

  26. Examination of Culture Growth • Potential pathogens • Slow growers • Growth on Mycosel • Color: dull buff, brown, mousy gray • Dimorphic

  27. Examination of Culture Growth • Growth rate • Rapid growers: 1-5 days • Intermediate growers: 6-10 days • Slow growers: >10 days

  28. Colony Morphology –Appearance • Rugose • Umbonate • Verrucose • Flat

  29. Colony Morphology –Texture • Cottony • Glabrous • Granular • Velvety

  30. Colony Morphology –Pigmentation • Surface • Reverse

  31. Microscopic Morphology • Definitive means of identification • Evaluate: • Shape • Method of production • Arrangement of conidia/spores • Size and color of hyphae

  32. Microscopic Techniques • Tease mount • Scotch tape preparation • Slide culture

  33. Serological Diagnosis • Immunodiffusion • Complement fixation • EIA • Latex agglutination

  34. Antifungal Susceptibility • Determine appropriateness • Standardization of testing • Methods • Predictability in vivo • Antifungal agents

  35. In Summary … • Specimen collection and transport • Specimen processing and culture • Direct examination of specimen • Examination of culture • Serological testing • Antifungal susceptibility

More Related