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The Effectiveness of Disinfectants on Pseudomonas fluorescens

The Effectiveness of Disinfectants on Pseudomonas fluorescens. Michelle Alvarez, Kadija Bangurah & Paige Stox Department of Microbiology North Carolina State University. Inspiration. How effective are your everyday household cleaners against bacteria?

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The Effectiveness of Disinfectants on Pseudomonas fluorescens

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  1. The Effectiveness of Disinfectants on Pseudomonas fluorescens Michelle Alvarez, KadijaBangurah & PaigeStox Department of Microbiology North Carolina State University

  2. Inspiration • How effective are your everyday household cleanersagainst bacteria? • Does it really kill 99% of germs?

  3. Pseudomonas fluorescens • Gram-Negative • Rod- Shaped • Multiple flagella • Soil and marine environments • Obligate aerobe • The optimum growth temperature is 25-30 degrees Celsius • Produces the antibiotic Mupirocinwhich treat skin, ear, and eye disorders • Nonpathogenic and lack virulence factors

  4. Problem/ Hypothesis • Problem: Which disinfectant will be the most effective on killing Pseudomonas fluorescens? • Hypothesis: Clorox Bleach Gel will be the most effective on killing Pseudomonas fluorescens.

  5. Method/Materials Series of Dilutions: • 1: 20 • 1: 30 • 1: 50 • 1: 100 • 1: 1,000 • 1: 10,000 • 1: 1,000,000 • Optimal Density is measured by the bioscreen C instrument for 60 hours (9) Disinfectants used: • Vinegar- Acetic Acid • Hand Sanitizer- ethyl alcohol • Clorox Gel Bleach- Sodium Hypoclorite • Pine Sol- Pine Oil • Hand Soap- Sodium Laureth Sulfate • Clorox Wipes- n- Alkyl-Dimethyl Benzyl Ammonium Chlorde • Lysol Spray- Hydrogen Peroxde • Dish Detergent- Triclosan • Shampoo- Ammonium Laureth Sulfate • Bioscreen C instrument • 10x Nutrient Broth • dH20 • Culture: Pseudomonas fluorescens

  6. Preliminary Trial 1

  7. 1:10,000 dilution

  8. 1:100,000 dilution

  9. Conclusion • The top two disinfectants that worked the best at inhibitingbacteria growth were Pine Sol and Clorox Bleach Gel. • At 1:100,000 dilution our culture started to grow and both disinfectants were no longer capable of killing our bacteria. Therefore our hypothesis was supported!

  10. Why? • Pine Sol: Pine Oil • Clorox Bleach Gel: Sodium Hypochlorite

  11. Questions?

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