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Lab 10- Colony isolation

Lab 10- Colony isolation . Mixed culture and unknown. Cultivation. The process of growing microorganisms in culture by taking bacteria from the infection site (in vivo or environment) and grow them in artificial environment in the laboratory (in vitro). Colony- (clone).

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Lab 10- Colony isolation

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  1. Lab 10- Colony isolation Mixed culture and unknown

  2. Cultivation • The process of growing microorganisms in culture by taking bacteria from the infection site (in vivo or environment) and grow them in artificial environment in the laboratory (in vitro).

  3. Colony- (clone) • Colony- A bacterial population derived from one bacterial cell. The cells within the colony have identical, genus, species, genetic and phenotypic characteristics. • Mixed culture- contains more than one type of colony • Pure bacteria/ culture - derived from a single colony.

  4. Isolation of bacteria - Dilution • Selection of a pure colony from a mixed culture -most important for bacterial identification • The inoculum is spread on the surface of an agar plate in such a pattern so that individual bacterial colonies are obtained. • Dilution of the original inoculum such that the colonies grow separate from each other

  5. Streak Plate technique

  6. Mixed culture

  7. Example 1: Good single colony isolation?

  8. Example 2: Good single colony isolation?

  9. Example 3: Good single colony isolation?

  10. Lab activities: • Streaking bacteria for isolation: • From a mixed culture / student • 2xNA plates /student @ 37oc • From unknown / student • NA, BHI, DSA @ 37oc • NA, BHI, DSA @ room temperature (RT)

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