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TEM GRID vs. DIME

TEM GRID vs. DIME. How do they look under microscope?. Square Grid. Hexagonal Grid. 200 mesh 55% open area, 28 µm bar width Thick:17.8 µm . 3 mm. TEM Grid Preparation. There are two sides in carbon coated ultra-thin grid

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TEM GRID vs. DIME

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  1. TEM GRID vs. DIME

  2. How do they look under microscope? Square Grid Hexagonal Grid • 200 mesh • 55% open area, • 28 µm bar width • Thick:17.8 µm 3 mm

  3. TEM Grid Preparation • There are two sides in carbon coated ultra-thin grid • Formvar side - lighter side (less hydrophobic than the carbon film) • Carbon film is on the darker side.

  4. TEM Grid Preparation • Hold the grid with tweezers. • Do not touch/contaminate the grid. • Do not bend the grid.

  5. TEM GRID PREPARATION • Take a micropipette and put a droplet on top the grid. Let the solution sit on the grid. • After 10-12 min, wick away the rest of the solution with filter paper. Do not touch the grid! • Finally let it sit until the grid dries off.

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