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Comprehensive Analysis of Plasmid pBR322 and Its Restriction Sites

This document presents an in-depth examination of the plasmid pBR322, focusing on its structure and the various restriction sites, including EcoR I, Hind III, BamH I, and others. The plasmid's important features, such as its ampicillin and tetracycline resistance genes, origin of replication, and size (4.36 kb), are thoroughly discussed. This analysis aids in understanding how to effectively use this plasmid in molecular cloning and genetic research, highlighting its applications in gene screening and manipulation.

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Comprehensive Analysis of Plasmid pBR322 and Its Restriction Sites

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  1. plasmid EcoR I 0 29 Hind III Xmn I 3966 ampr 375 BamH I tetr Pst I 3612 650 Sal I pBR322 4.36kb condition 1424 Ava I A origin Ascreening gene 2034 Xmn I ori 2067 Pvu II A single restriction site

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