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Technology of Engineering Food

This overview delves into the fascinating world of biotechnology, particularly in food engineering. Highlighting the genetic engineering of tomatoes with an antifreeze gene from flounder, it introduces key concepts like DNA structure, gene mechanisms, and transformation techniques. Various methods such as biolistic transformation and vector-mediated insertion are discussed, including the significance of marker genes. As we look to the future of biotechnology, we ponder the limits of scientific advancement and the potential for innovative solutions in agriculture and food production.

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Technology of Engineering Food

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  1. Technology of Engineering Food

  2. The common between... flounder tomato Tomato has an engineered gene from the flounder  Antifreezing gene

  3. Survey • What is biotechnology? - What is DNA? - What kind of mechanisms do exist? - What is the best method?

  4. Biotechnology • -What is Biotechnology? • What is DNA? • What kind of mechanisms do exist? • What is the best method?

  5. What is Biotechnology? • What is DNA? • What kind of mechanisms do exist? • What is the best method? DNA Shortening of: deoxiribonucleic acid -Segments of DNA are called genes -Four bases (Guanin, Cytosin, Adenin, Thymin) -Bases are connect with sugar and phosphat Double-helix-structure (like a spiral staircase)

  6. Mechanisms • What is Biotechnology? • - What is DNA? • - What kind of mechanisms do • exist? • What is the best method? Biologically Mechanically Vector: Bacterium Injection Biolistic Vector: Virus Protoplast Transformation

  7. Agrobacterium Tumefaciens

  8. Virus

  9. Protoplast Transformation - Taking a cell from an organism • Dissolving of the cellwall by enzymes •  Protoplast is left over - Adding the new DNA • Cellwall has to recover in a growth media

  10. Injection Injecting new DNA with a needle into the nucleus of a cell.

  11. Biolistic • Small beads coated with DNA are load in a gene gun. • Shooting the ball into the cell nucleus • It cuts the DNA section of the cell and „glues“ the desired DNA into it.

  12. Marker Genes • To find out if the transformation has been succesfull • scientists intodruce a marker gene to the host. • Cells which have been transformed will grow in a media while the other cells will die.

  13. Best Method • What is Biotechnology? • - What is DNA? • What kind of mechanisms do exist? • What is the best method?

  14. Isn´t it fantastic what the scientists can do with their technology?

  15. But what will the future bring? Do scientists know their limits?

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