Item Analysis • Question • Topic • Targets • Why incorrect? • Changes in strategy?
Enrico Fermi • Noted for his powers of estimation based upon little or no data
Distribution of Microorganisms Follow-Up • 1) Wear goggles • 2) Retrieve your two petri dishes and open them • 3) Using a stereoscopic microscope, observe the four quadrants of your dishes and assess the following for each quadrant/sample: • Number of Different Colonies/Strains of Monera • Estimated % of Quadrant Covered By Moneran Growth • Qualitatively Assess For Evidence of Fungal Growth • 4) When finished assessing the plates, tape them shut and dispose in lined garbage can • 5) Wash hands thoroughly
Analysis Paragraph For Distribution of Microorganisms Lab • Based upon your observations, what environmental conditions are required to foster the growth of bacteria? • What evidence do you have to support this contention? • Cite both qualitative and quantitative evidence. • Also, support your contentions by citing results from environments that lack these conditions. What conditions are prohibitive to microbial growth? • Distinguish between evidence of Moneran and Fungal growth. Were the conditions that fostered Moneran growth the same as those of Fungal growth? Were the prohibitive conditions the same? Compare and contrast these kingdoms in terms of their characteristics • Based upon your results, explain how methods of food preservation both modern and ancient (salting, smoking, icebox/refrigerator) correlate with the findings of your lab
Kirby-Bauer Antibiotic Sensitivity Follow-Up • 1) Obtain goggles, rulers, a laptop and several yellow inoculating loops • 2) Obtain your two plates from the table • 3) For each of the antibiotic discs, record the diameter in mm (to the nearest 0.5mm) of the zone of inhibition (if any). If the zone is irregular in size (non-circular), make your best estimate. • 4) Report your results to the front board ASAP • 5) Using the inoculating loops, remove the agar from the plate, remove any labels, and place in the bleach water tub on the central island.
Kirby-Bauer Formal Lab Report • Title • Purpose • Research • Antibiotic efficacy and specificity (from internet research) • Types of Bacteria Utilized (from handout) • Hypothesis: Based upon the information we have and the strains we are testing, which antibiotics should be most effective against which Monera? Why? • Data Table: Zones of Inhibition, Qualitative Data (i.e. evidence of resistant strains) • Conclusion: • Confirm/Refute your hypothesis • Which Monera were most/least sensitive • Which antibiotics were broad spectrum/narrow spectrum • Did any antibiotics display evidence of resistant strains • Sources of Error