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Purple Non Sulfur Bacteria

Purple Non Sulfur Bacteria. Hopkins Microbiology Course Ann Lesnefsky 7-15-2011. Metabolism of PNS Bacteria. Organic acid electron donor Photosynthesis for energy generation Bacterial chlorophyll b Absorbs light in the infrared range

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Purple Non Sulfur Bacteria

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  1. Purple Non Sulfur Bacteria Hopkins Microbiology Course Ann Lesnefsky7-15-2011

  2. Metabolism of PNS Bacteria • Organic acid electron donor • Photosynthesis for energy generation • Bacterial chlorophyll b • Absorbs light in the infrared range • Currently PNS bacteria are being studied for H2 production

  3. Course Enrichment Method for PNS • 6-18-2011 • Plated samples from the slough on succinate and ethanol plates, incubated anaerobically with light • Course’s media and Holly’s media • 6-29-2011 • Restreak single colonies for isolation

  4. TA Enrichment Method for PNS • TA’s enriched for PNS by filtering • Filter both water above mat and slurry of mat material • Place filter face down on plate • Flip and all allow PNS bacteria to grow on filter

  5. Microscopy of PNS • Colonies isolated from 5 different plates • 4 plates from strawberry • 1 from Kerby Park • All looked like stubby rods

  6. Recommendations • Next year use the filter method.

  7. Questions?

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