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This study investigates the impact of disrupting atSR45a on chlorophyll content and PSII activity in Arabidopsis plants under high-light stress. Two-week-old seedlings were subjected to high-light intensity (800 µmol m-2 s-1) for 6 hours. Measurements were taken for PSII activity (Fv/Fm), chlorophyll content, and phenotypic changes in both wild-type and KO-sr45a plants. Results highlight significant differences in stress tolerance associated with atSR45a disruption, informing future research on plant stress responses.
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A WT KO-sr45a 6 (h) 0 1 2 3 B C Chlorophyll (µg mg FW-1) PSII activity (Fv/Fm) Time (h) Time (h) Fig. S1
Supplementary Fig. S1. Effects of disruption of atSR45a on high-light stress tolerance. Phenotypes (A), PSII activity (Fv/Fm)(B), and chlorophyll contents (C) of the wild-type and KO-sr45a plants under high-light stress. Two-week-old seedlings were exposed to high-light intensity (800 µmol m-2 s-1) for 6 h. The PSII activity (Fv/Fm) in the leaves of Arabidopsis plants was determined at 25˚C after adaptation to the dark for 30 min. Data are the mean ± SD for three individual experiments (n=3). FW, Fresh weight.