VCN 0.32 VCN 0.41 VCN 2.1 VCN 0.15 VCN 0.22 VCN 0.38 VCN 0.05 VCN 0.22 VCN 0.45 Figure S2: A2UCOE gives rise to stable eGFP expression in P19 cells compared to SFFV and EF1α. A % EGFP expression D3 D7 D11 D14 D18 B % EGFP expression D3 D7 D11 D14 D18 C % EGFP expression D7 D15 D19 D3 D24 P19 cells were transduced with different concentration of virus. eGFP expression was assessed by FACS at different time points. A: cells transduced with LV-SFFV-eGFP vector; B: cells transduced with LV-EF1α-eGFP vector; C: cells transduced with LV-UCOE-eGFP vector. VCN: vector copy number per cell determined by Q-PCR. A declined EGFP expression occurs in cells transduced with LV-SFFV-eGFP and LV-EF1α-eGFP vectors at the different concentration of viruses. However, a stable eGFP expression is remained in cells transduced with LV-UCOE eGFP vector at different concentration of virus.