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prrF1 TTGACATGGA AATGAGAATC ATTATTATGT CACTCAACTG GTCGCGAGAT CAGCCGGTAA

prrF1 TTGACATGGA AATGAGAATC ATTATTATGT CACTCAACTG GTCGCGAGAT CAGCCGGTAA prrF2 TTGACCTGAT AATGAGAATA GTTATTATTA CAC-CAACTG GTCGCGAGGC CAGCAGGTAA arrF TTGACCACAA AATGAGAAGC GTTATTATTT GA--AAACTG ATCGCGAGAT CAGC-GGTAA prrF1 GCTGAGAGAC CCACGCAGTC GGACTCTTCA GATTATCTCC TCATCAGGCT AATCACGGTT

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prrF1 TTGACATGGA AATGAGAATC ATTATTATGT CACTCAACTG GTCGCGAGAT CAGCCGGTAA

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  1. prrF1TTGACATGGA AATGAGAATC ATTATTATGT CACTCAACTG GTCGCGAGAT CAGCCGGTAA prrF2TTGACCTGAT AATGAGAATA GTTATTATTA CAC-CAACTG GTCGCGAGGC CAGCAGGTAA arrFTTGACCACAA AATGAGAAGC GTTATTATTT GA--AAACTG ATCGCGAGAT CAGC-GGTAA prrF1GCTGAGAGAC CCACGCAGTC GGACTCTTCA GATTATCTCC TCATCAGGCT AATCACGGTT prrF2GCTGAGAGAC C-AAGCAGTC GGACTCTTCA GATTATCTCC TCATCAGGCT AATCACGGTT arrFACTGACAGA- ----GCGGCT --ACTCTTCA GATTATCTCC TCATCAGGCT AATCACGGTT prrF1TTTGACCCGG CACTTTGCCG GGTCTTTTTT T prrF2TC-GACCCGG CACTTTGCCG GGTCTTTTTT T arrFTTTGACCCGG CTCG--GCCG GGTCTTTTTT T -35 -10 Fur Box Core Sequence Supplemental Figure 3. Nucleotide sequence alignment of the arrF, prrF1 and prrF2 genes. Sequence alignments were generated using ClustalW. Iron box, the central core sequence, and predicted -35 and -10 promoters are indicated in bold line and labeled on the top or bottom of the sequence.

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