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Plant Tissue Culturing Problem Scenario 3A

Plant Tissue Culturing Problem Scenario 3A. Problem Scenario Overview. Challenged with designing a plant manufacturing system capable of producing food by means of tissue culturing. System Requirements. Limited space (approx 2’x1’x1’) Light source

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Plant Tissue Culturing Problem Scenario 3A

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  1. Plant Tissue Culturing Problem Scenario 3A

  2. Problem Scenario Overview • Challenged with designing a plant manufacturing system capable of producing food by means of tissue culturing.

  3. System Requirements • Limited space (approx 2’x1’x1’) • Light source • Automated temp. control, light exposure, nutrients. • Allow for increase in plant size • Sealed sterile environment, allowing for manipulation of plants and tools

  4. Biological Information • Boston fern or “Nepholepis exaltata” was chosen for this problem scenario because it is a variety that is easy to culture, thus making it appropriate for learning tissue culturing techniques.

  5. Organism Selection • May be based on any number of criteria, depending on the reasons for culturing • may include the plants ability to provide food • profit from sale of the plant • preservation of an endangered or threatened species • selection may also be based on the ability of the plant to be cultured, and/or the intended +method of culturing

  6. Life Requirements of System • Need to address: • Temp • Light • Air/CO2 • Water/Humidity • Nutrients/pH • Sterile environment

  7. Technical Information • This system will accommodate all the environmental requirements of this organism because: • clean room by definition had a low level of environmental pollutants, such as dust, airborne microbes, aerosol particles and chemical vapors. • Our system supplies nutrients, a sterile environment and enough physical space to develop.

  8. Pros Clean plants of infection and quickly regenerate Produce in absence of seeds Conserve rare/endangered species Reproduce stress tolerant strains Cons Too many of a narrow genetic strain may limit gene pool of natural population Threatens traditional faming techniques Expensive/equipment intensive Some varieties are difficult to culture Impacts of Tissue Culturing (pros and cons)

  9. Computer Control Opportunities • Some potential mechanism that could be used to monitor, run, and maintain the system would be: • *Differential pressure gage • * ThermoHygrometer (temp & humidity) • *Air velocity monitor • *pH meter • *Light sensor

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