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Glutathione S- transferase

Glutathione S- transferase. By : Ira P rima Sari (20512047) Relita Pebrina (20512025). Pendahuluan.

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Glutathione S- transferase

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  1. Glutathione S-transferase By : Ira Prima Sari (20512047) RelitaPebrina (20512025)

  2. Pendahuluan • Glutathione (GSH)adalahtripeptidadenganikatan gamma peptide antaragugusaminadarisistein (yang terikatolehikatan peptide padaglisin) dankarboksildarirantaisampingasamglutamate • GSH merupakanantioksidan yang mencegahkerusakankomponenselpenting yang disebabkanolehspesiesoksigenreaktifsepertiradikalbebasdanperoksida • Reaksikonjugasidanpenguranganreaksi glutathione dikatalisisolehenzimGlutathione S-transferase (GSTs) dalamsitosol, mikrosomdanmitokondria.

  3. StrukturdanTatanama GSTs • Menurut IUBMB : EC 2.5.1.18 • Glutathione S-transferase (GSTs)adalahenzimdimer yang ditemukanpadamamalia, serangga, tanaman, danmikroba yang mengkatalisisserangannukleofilikoleh anion tiolat GSH dielektrofilikpusatmolekulhidrofobik (Mannervikdan Danielson, 1988).

  4. Struktur GSTs

  5. Klasifikasi GSTs

  6. Isolasi GSTs • GST banyakterdapatpadasitosol, mitokondria, danmikrosomal protein padaspesieseukariotikdanprokariotik • Pemurniannyabisadidapatdarihewansepertisapimenggunakanafinitas, pertukaran ion, hidrofobikdankromatografifiltrasigel, tetapimetodepemurnian yang paling efektifadalahafinitaskromatografipada glutathione-agarosematriks

  7. AplikasidanFungsi GSTs • Glutation S-transferaseadalahenzimmultifungsikompleks yang berperanpadadetoksifikasisenyawaelektrofilikxenobiotik

  8. Modifikasi GSTs • ikatan yang stabilantaracyPGdanGSTP1-1dandapatdigunakansebagaiperspektifbaruuntukpengembangan inhibitor GSTireversibelsebagaiagenantikanker.

  9. Thank you

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