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Characterization of Vpx-VLPs and Their Impact on Infectious Lentiviruses in GFP+ Cells

This study explores the role of Vpx-VLPs in enhancing the infectivity of HIV-2 and SIVMAC in GFP+ cells. We assess the effects of pre-incubation protocols on viral entry using infectious lentiviral vectors. Different strains, including HIV-2, SIVMAC, and SIVRCM, were tested at varying multiplicities of infection (MOI). The presence of the CA p27 protein and Flag-Vpx was analyzed to understand the mechanism of Vpx's contribution to viral functionality. Findings reveal significant insights into the viral lifecycle and potential therapeutic targets.

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Characterization of Vpx-VLPs and Their Impact on Infectious Lentiviruses in GFP+ Cells

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  1. A no pre-inc. 100 Vpx-VLPs pre-inc. 10 % GFP+ cells 1 0,1 - - + + Vpx: Infectious LV: HIV-2 SIVMAC B Infectious LV: SIVMAC Cell Virus 100 MOI 0.3 3 CA p27 Flag-Vpx 10 - - % GFP+ cells HIV-2 HIV-2 SIVMAC SIVRCM SIVMAC SIVRCM 1 Flag-Vpx Origin 0,1 SIVMAC - SIVRCM HIV-2 Flag-Vpx: C Infectious LV: HIV-1 100 Cell Virus CA p27 Flag-Vpx % GFP+ cells 10 HIV-2 HIV-2 SIVMAC SIVRCM SIVMAC SIVRCM Flag-Vpx Origin 1 SIVMAC Flag-Vpx-VLPs pre-inc. - SIVRCM HIV-2

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