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San Diego Epigenome Center An NIH Reference Epigenome Mapping Center (REMC)

San Diego Epigenome Center An NIH Reference Epigenome Mapping Center (REMC). Antibody Screening. Antibody Screening. Screened 84 antibodies against 47 marks 77 dot blots are currently posted. Of those:. *does not bind other peptides (potentially specific).

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San Diego Epigenome Center An NIH Reference Epigenome Mapping Center (REMC)

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  1. San Diego Epigenome CenterAn NIH Reference Epigenome Mapping Center (REMC) Antibody Screening

  2. Antibody Screening Screened 84 antibodies against 47 marks 77 dot blots are currently posted. Of those: *does not bind other peptides (potentially specific) Now have ChIP-grade antibodies for 29 marks Have generated ChIP-Seq data for 25 marks

  3. Dot Blot Layout Each peptide is spotted at two different concentrations Specificity is calculated based on spot intensity

  4. Specific Dot Blot Single band on Western (WCE)

  5. H3K9Me1 1 = H3K9Me1 (100%) Diagenode: Cat#: pAb-065-050 Lot #: A89-0041 1 16.2

  6. H3K9Me2 1 = H3K9Me1 (5.5%) 2 = H3K9Me2 (94.5%) Diagenode: Cat #: pAb-060-050 Lot #: A90-0041 1 21.2

  7. Specific Dot Blots Two bands on Western(WCE)

  8. H3K4Me1 1 = H3K4Me1 (100 %) Catalog #: Ab8895 Lot #: 720417 1 5.4

  9. H3K18Ac 1 = H4K8Ac (0.5 %) 2 = H3K18Ac (99.5 %) Catalog #: Ab1191 Lot #: 527530 1 2 17.2

  10. Non-specific Dot Blot Multiple spots Clean Western

  11. H3K79Me3 1 = H3K79Me3 (66.7%) 2 = H3R17Me2as (10.0%) 3 = H3K79Me2 (24.3%) Diagenode: Cat #: pAb-068-050 Lot #: A86-050 1 2 3 1.2

  12. H3K27Ac 1 = H3K9Ac (18.8 %) 2 = H3K27Ac (78.5 %) 3 = H3K56Ac (1.3 %) 4 = H3K18Ac (1.2 %) 5 = H2BK5Ac (0.3 %) Catalog #: Ab4729 Lot #: 726657 1 2 3 4 5 4.5

  13. Non-specific Dot Blot WRONG Epitope

  14. H3K9Ac 1 = H3K9Ac (6.7 %) 2 = H3K56Ac (93.3 %) Catalog #: Ab4441 Lot #: 677405 1 2 19.4

  15. H3R3Me1 1 = H3R17Me2as (93.6 %) 2 = H3R17Me2s (6.4 %) Catalog #: Ab17339 Lot #: 396016 1 2 27.1

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