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Influence of Triton X-100 Concentration on Sterol Enrichment and LCB Composition

Study on the impact of different Triton X-100 concentrations on sterol enrichment and LCB composition in 100 µg protein equivalents. Results presented include qualitative and quantitative data on various sphingolipids.

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Influence of Triton X-100 Concentration on Sterol Enrichment and LCB Composition

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  1. (A) (B) AdditionalFigure A1 : Influence of Triton X-100 concentration: 3:1, 3:2, 6:1 and 6:2 (detergent/protein ratio:final detergent concentration) on (A) sterol enrichment and (B) quantitative and qualitative composition of LCB from 100 µg protein equivalents. Abbreviations used: t18:1(8Z): 4-hydroxysphing-8(Z)-enine, t18:1(8E): 4-hydroxysphing-8(E)-enine, t18:0: 4-hydroxysphinganine (phytosphingosine), d18:1(8Z): sphing-8(Z)-enine, d18:1(8E): sphing-8(E)-enine, d18:0: sphinganine (dihydrosphingosine), d18:2(4E,8E,Z): sphinga-4(E),8(E,Z)-dienine, d18:1(4E): 4-hydroxysphinganine. Results relative to sterol enrichment are expressed as the means ± SE (vertical bars) of at least three independent preparations.

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