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H.Ghaderian1

H.Ghaderian1 1-Deparyment of Microbiology , Faculty of Biological Sciences , Islamic Azad Univercity , Falanarjan Branch , 804515/155 , Esfahan , Iran. : Classification. Family : Herpes viridea Sub family : herpes virinea Genus : simplex Specieas:HSV-1 CL 101 ,HSV-2 strain KN 53690

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H.Ghaderian1

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  1. H.Ghaderian1 1-Deparyment of Microbiology , Faculty of Biological Sciences , Islamic Azad Univercity , Falanarjan Branch , 804515/155 , Esfahan , Iran

  2. :Classification • Family : Herpes viridea • Sub family : herpes virinea • Genus : simplex • Specieas:HSV-1CL 101 ,HSV-2 strain KN 53690 common name : herpes symplex virus

  3. tegument

  4. Alpha herpes viruses latently infected

  5. Herpes virus in the host cell

  6. HSV of the newborn is acquired 3 periods: in utera Peripartium (85%) postpartum

  7. Incidence of neonatal herpes in 3 form : 1) lesion of the skin , eyes , mouth

  8. 2) Encephalitis with or without lesion

  9. 3) Dissiminateddesease in liver lunges gland adrenl gut

  10. Neonatal herpes • caseof neonatal herpesHSV -1and HSV-2 • Investigation showed Neonatal disseminated HSV infection is most frequently caused by HSV-2, although HSV-1 can also be the cause • Serologic investigations showed noIgMand IgGantibodies forHSV-1 and HSV-2 in new born

  11. Immune ResponsesNeonatal Infected neonates produce HSV-specific IgM antibodies within 3 weeks of acquisition of the viral infection, which increaserapidly during the first 2 to 3 months and may remain for as long as 1 year after neonatal infection develops.

  12. Lymphocytes from infected infants alpha-interferon NeonatesINF –αcompare with adults with primary HSV infection is decreased.

  13. Study showed : The first time infection of the pregnantwomen may lead to severe illness in pregnancy and may be virus transmission to newborn.

  14. DIAGNISTIC 1)PCR The high risk for death requires prompt diagnostic therforesuggestive that of HSV was identified by PCR Viral DNA was extracted from all specimens (tracheal aspirate, liver, lungs, and gut) By using the QIAamp DNA Mini Kit

  15. primers for the HSV-1: thymidinekinasegene ((Fw 5′-AGCGTCTTGTCATTGGCGAA-3′ (Rev 5′-TTTTCTGCTCCAGGCGGACT-3′) Primer for the HSV-2: DNA polymerase gene ((Fw 5′-CGTCCTGGAGTTTGACAGCG-3′ (Rev 5′-CAGCAGCGAGTCCTGCACACAA-3′) The DNA was then used for HSV DNAPCR

  16. Nucleotide sequence analysis showed identical sequences in different specimens. GenBank BLAST tool highest similarity was HSV-1 strain CL 101 and HSV-2 strain KN 53690

  17. 2)Cell culture • Multi nucleatedepithelial cells

  18. treatment • All infants with diagnosed HSV infection must be treated with an intravenous therapy with acyclovir (60 mg/kg/day) • Antibody Therapy Antibodies against the surface glycoproteins gB and gDprophylactic and therapeutic agents inHSV infection

  19. mechanism acyclovir inactive acyclovir Viral thimydin kinas Monophosphteacyclovoir cellular thimydin kinase active ))acyclovir three phosphate is analog to guanin blocked DNApolymerasefunction

  20. PREVENTION Cesarean Delivery cesarean delivery in a woman with active genital lesions can reduce risk of acquiring HSV to infant Antiviral Prophylaxis During Pregnancy the use of oral acyclovir near the end of pregnancy to suppress genital HSV Because of acyclovir’s safety

  21. Hsv vaccine HSV-2 : glycoprotein D subunit vaccine adjuvanted :monophosphorylipid A(MPL) efficacy was limited to women who were HSV-1 and -2 seronegative before receiving vaccination.

  22. Thank you for your Attention

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