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Impact of Antibiotics on Yeast Survivorship Study

Investigating the effects of antibiotics on yeast survivorship using Saccharomyces cerevisiae as a model organism. The experiment involves culturing yeast with varying concentrations of antibiotics and antifungals to observe their impact on survivorship.

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Impact of Antibiotics on Yeast Survivorship Study

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  1. The Effects of Antibiotics on Yeast Survivorship Chawrley Curr Grade 10 Pittsburgh Central Catholic High School

  2. Candidiasis • Commonly known as yeast infections • Caused by uncontrolled multiplication of yeast cell Candida albicans in body • Candida albicans can live in human mouths and in the gastrointestinal tract without causing harm • Antibiotics and steroids use are most common reason for yeast infections Oral Yeast Infection

  3. Nystatin • Antifungal drug • Used for treatment of yeast infections of the stomach, mouth, intestine and mucus membranes • Attaches to ergosterol which is a major component of cell membrane • Ergosterol is unique to fungi so nystatin does not have a catastrophic impact on animals

  4. Saccharomyces cerevisiae • Eukaryotic • Reproduces by budding • Most commonly studied eukaryotic model organism • Top-fermented yeast

  5. Ampicillin • Penicillin antibiotic • Used in treatment of bacterial and fungal infections • Acts as competitive inhibitor of an enzyme and inhibits the final stage of cell wall synthesis and causes cell lysis

  6. Materials: • 60 YEPD agar plates ( • Klett spectrophotometer • Sterile pipette tubes • Micropipettes • 4 one liter beakers • Vortex • Incubator • Sidearm flask • Spreading platform • Spreader bar • Ethanol • 20 mL Sterile capped test tubes with sterile dilution fluid (SDF) • Ampicillin • Nystatin Suspension

  7. Procedure: • Yeast was grown overnight in sterile YEPD agar media • A sample of the overnight culture was added to fresh media in a sterile sidearm flask. • The culture was placed in a shaking water bath (30°C) until a density of 50 Klett spectrophotometer units was reached. This represents a cell density of approximately 107 cells/ml • The culture was diluted in sterile dilution fluid to a concentration of approximately 107 cells/ml • The selected experimental variables were diluted with sterile dilution fluid to the chosen concentrations to a total of 9.9mL • 0.1 mL of cell culture was then added to the test tubes, yielding a final volume of 10 mL. and a cell density of approximately 103 cells/mL • The solution was mixed by vortexing and allowed to sit at room temperature for 15 minutes • After vortexing to evenly suspend cells, 0.1 mL aliquots were removed from the tubes and spread on 60 plates • The plates were incubated at 30°C for 12 hours. • The resulting colonies were counted. Each colony is assumed to have arisen from one cell.

  8. Purpose: • To determine how various concentrations of antibiotics and antifungals affect yeast survivorship Hypothesis: • Increasing concentrations of antibiotics and antifungals will result in the decrease of yeast survivorship Null Hypothesis: • There will be no significant variation in yeast survivorship at all concentrations

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