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Effects of low magnetic fields on T1values for hyperpolarized 13 C Pyruvic Acid

Effects of low magnetic fields on T1values for hyperpolarized 13 C Pyruvic Acid. Bryan Addeman April 6, 2010. Agenda. Background Theory Motivation Measurements Moving Forward. Why use 13 C instead of 1 H?. Label specific metabolites with 13 C 12 C isotope is not magnetically active.

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Effects of low magnetic fields on T1values for hyperpolarized 13 C Pyruvic Acid

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  1. Effects of low magnetic fields on T1values for hyperpolarized 13C Pyruvic Acid Bryan Addeman April 6, 2010

  2. Agenda • Background Theory • Motivation • Measurements • Moving Forward

  3. Why use 13C instead of 1H? • Label specific metabolites with 13C • 12C isotope is not magnetically active

  4. What is hyperpolarization?

  5. Why does the sample need to be hyperpolarized? • Natural abundance of 13C is ≈ 1.11% • Amount of signal is related to gyromagnetic ratio (γ) & abundance • γH = 42.58 (Mhz/T) • γ13C = 10.71 (Mhz/T) • Hyperpolarization can give a factor of 10,000x more initial signal

  6. Hyperpolarization Process • Inject sample mixed with free radical • Cool to 1.4 K in a High magnetic field • Bombard sample with microwaves • Mix with heated solution • Duration: 1.5 hours • Amount of Hyperpolarization decays with time (T1) Robarts DNP Hyperpolarizer

  7. The T1 Relaxation Process T2 T1 Spin-Spin Relaxation Spin-Lattice Relaxation

  8. Pyruvic Acid

  9. Effects of low magnetic fields on T1values for hyperpolarized 13C Pyruvic Acid

  10. Motivation • Hyperpolarized sample transport: • Bo: High (DNP) -> Low -> High (MRI) • What happens to the sample in transit? • T1 relaxation is a characteristic of the molecule that can be measured

  11. Measuring T1 • Variable magnetic field • Bo = 0.0-0.9 T • Desktop NMR system • Bo = 0.55 T • Both devices can measure Signal amplitude at time intervals Spinmaster Oxford Instruments MQC

  12. Analysis • Fit Equation • Non-Linear Least Squares Fit Bo=0.55, 13C enriched Pyruvic Acid

  13. Results

  14. Conclusions • Need to take more measurements to understand the effects low field strengths on T1 values

  15. Acknowledgments Supervisors • Dr. Charles McKenzie • Dr. Lanette Friesen-Walder • Dr. Tim Scholl • Dr. Jake Onwuachi Lab • Curtis Wiens • Samantha Flood • Steven Yange • Elmer Soto • Trevor Wade

  16. Questions

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