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Understanding Recombinant DNA Technology and Genetic Engineering Concepts

This article explores the fundamental concepts of recombinant DNA technology and genetic engineering. It outlines the general procedures involved, including cutting, linking, and transfection. The discussion includes various vectors and sources of foreign DNA, such as genomic DNA, complementary DNA (cDNA), and synthetic fragments. Key techniques like restriction endonuclease digestion, microinjection, and transgenic methods are explained. The article also covers essential screening methods such as drug-resistance gene identification and Southern blotting for effective gene analysis.

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Understanding Recombinant DNA Technology and Genetic Engineering Concepts

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Presentation Transcript

  1. DNA recombinant technology & genetic engineering (1) several concepts * recombinant DNA * technology of recombinant DNA

  2. * Genetic engineering • general procedure • A. separate • B. cut • C. linkage • D. transfection or • transformation

  3. E. Screening a. separate * Vectors concept type

  4. * the sources of foreign target DNA three methods genomic DNA complementary DNA,cDNA synthetic DNA fragment Lehninger 112601

  5. Genomic DNA library cDNA library • b. cut • restriction endonuclease • concept • nomination • type

  6. 5’ G-A-A-T-T-C 3’ 3’ C-T-T-A-A-G 5’ 5’ G 3’ 5’ A-A-T-T-C 3’ 3’ C-T-T-A-A-5’ 3’ G 5’ sticky end

  7. 5’-A-G-C-T-3’ 3’-T-C-G-A-5’ 5’-A-G-3’ 5’-C-T-3’ 3’-T-C-5’ 3’-G-A-5’ blunt end

  8. Linkage • d. transformation or transfection • microinjection • transgenic • E. Screening • drug-resistance gene • X-gal screening

  9. Southern blot *digested with enzyme * electrophoresis * transfer * hybridization * autoradiography

  10. Lehninger 92201

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