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Investigating the Anti-fungal Properties of Subterranean Termites

Investigating the Anti-fungal Properties of Subterranean Termites . Yasamin Sharifzadeh , Katie Gorick , Wong Hong Jie , Samuel Lau. Background . Our project. Objectives Hypothesis Overview of project. Objectives. Overview. American vs Asian. Hypothesis.

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Investigating the Anti-fungal Properties of Subterranean Termites

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  1. Investigating the Anti-fungal Properties of Subterranean Termites YasaminSharifzadeh, Katie Gorick, Wong Hong Jie, Samuel Lau

  2. Background

  3. Our project Objectives Hypothesis Overview of project

  4. Objectives

  5. Overview American vs Asian

  6. Hypothesis

  7. Methodology Body fluid extraction (Total system) Hemolymph extraction Hemolymph w/o protozoa and bacteria Hemolymph w/ bacteria but no protozoa Anti-fungal assay

  8. Body fluid extraction (Total system)

  9. Body fluid extraction (Total system) • Wash the termites in a beaker • Filter termites with a sieve and repeat washing for 2 more times • Crush the termites with a homogenizer • Homogenize using the phosphate buffer solution • Centrifuge to pellet tissue debris • Collect the supernatant • Place the supernatant in micro centrifuge tubes • Centrifuge at 1300rpm for 10 minutes • The body fluids are now separated • Extract the supernatant and filter it with a micro-filter

  10. Hemolymph extraction

  11. Hemolymph extraction

  12. Hemolymph extraction (cont’d)

  13. Hemolymph (cont’d)

  14. Anti-fungal assay

  15. Anti-fungal assay

  16. Anti-fungal assay

  17. Anti-fungal assay

  18. Timeline

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