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Understanding the Role of Puroindolines in Wheat Hardness

Puroindolines a and b genes are crucial for determining wheat hardness. The absence of these genes leads to hardness, while their presence characterizes soft wheats. The PCR band for the "soft" allele along with puroindoline a signifies soft wheats, whereas an absence indicates a puroindoline b mutation in soft wheats. This study explores the genetic link between wheat hardness and puroindolines.

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Understanding the Role of Puroindolines in Wheat Hardness

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  1. Puroindolines ‘a’ and ‘b’ are completely linked with hardness Puroindolinea Puroindoline b • Absence of this gene is always • associated to hardness • Presence of the PCR band for the “soft” allele combined with presence of the puroindolin a is characteristic of a soft wheats • Absence is always associated with • a puroindoline b mutation present • in soft wheats BC1 Express / 2*UC1036 BC1 Express / 2*UC1036 BC1 Express / 2*UC1036 BC1 Express / 2*UC1036 Yecora Rojo (Hard) Cheyenne (Hard) Express (Hard) UC1036 (Hard) Yecora Rojo (Hard) CS (Soft) Express(Hard) UC1041 (Hard) UC1036 (Hard) UC1037 (Hard) Anza (Hard) RSI5 (Hard) CS (Soft) Hae III- MTA9 RFLP Puroindoline a PCR of puroindoline b sequence for the “soft” allele (Giroux & Morris TAG, 1997)

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