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Sinteza RNA

Sinteza RNA. Voet : poglavji 31.1 in 31.2 Stryer 5: poglavje 28 Krebs 3: poglavji 19 in 20. Holoencim : 460 kDa ; brez podenote sigma: ‚osrednji encim‘. footprinting = iskanje odtisov. http://www.pingrysmartteam.com/. transkripcijski mehurček (brez dela podenote beta):

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Sinteza RNA

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  1. Sinteza RNA • Voet: poglavji 31.1 in 31.2 • Stryer 5: poglavje 28 • Krebs 3: poglavji 19 in 20

  2. Holoencim: 460 kDa; brez podenote sigma: ‚osrednji encim‘.

  3. footprinting = iskanje odtisov

  4. http://www.pingrysmartteam.com/ transkripcijski mehurček (brez dela podenote beta): aktivacijski kompleks prokariontske RNA-polimeraze

  5. http://porpax.bio.miami.edu/~cmallery/150/gene/sf13x5a.jpg

  6. RNA-polimeraza Taq(holoencim) v kompleksu s promotorsko regijo na DNA.

  7. http://porpax.bio.miami.edu/~cmallery/150/gene/sf13x5b.jpg

  8. Alberts 4

  9. Hipotetičen močan terminator E. coli.

  10. RNaza P (E. coli): M1 + C5 M1: RNA C5: polipeptid http://sandwalk.blogspot.com/2008/01/mondays-molecule-58.htmlhttp://sandwalk.blogspot.com/2008/01/transfer-rna-processing-rnase-p.html

  11. Science 328, 501 (2010)

  12. Podenote RNA-polimeraz pri pro- in evkariontih.

  13. ArikDvir, Oakland Univ., Biol. Sci.

  14. Regulacijski elementi cis in trans • cis: del iste verige DNA, ki jo uravnava (npr. promotorji, ojačevalna zaporedja) • trans: prihajajo od drugod (npr. transkripcijski faktorji) Transkripcijski faktorji se sicer vežejo na ojačevalna zaporedja, a so lahko kodirani na drugih kromosomih.

  15. http://www.mun.ca/biochem/courses/3107/images/eukprom.GIF

  16. Alberts 4

  17. Nature Reviews Molecular Cell Biology 3, 702-710 (2002)

  18. število podenot RNAPII: 12 TFIIH: 9 TFIIE: 2 TFIIF: 3 TFIID: 14 SRB/mediator: >16 Swi/Snf: 11 SRB10 CDK: 4 SAGA: 13 Iniciacija transkripcije z RNA-pol II pri kvasovki Sodeluje 10 proteinskih kompleksov s skupaj >85 proteini.

  19. http://web.wi.mit.edu/young/pub/RNAPolymerase.html

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