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Figure S1. LNCaP. LY294002. -. +. +. Hours. -. 12. 24. p-AKT. AKT. p53. p21. ß-actin. Figure legend S1: LNCaP cells were treated with the PI3K inhibitor LY294002 ( 20 m m ) for the indicated time points.
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Figure S1 LNCaP LY294002 - + + Hours - 12 24 p-AKT AKT p53 p21 ß-actin Figure legend S1: LNCaP cells were treated with the PI3K inhibitor LY294002 (20 mm) for the indicated time points. Immunoblots were probed for antibodies targeted to p-AKT, AKT, P53, P21 and actin. Treatment with LY294002 causes inhibition of the phosphorylation of AKT (Ser 473). There is no change in the levels of P53 protein.