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RATS

RATS. Usually very friendly and accept handling and gentle manipulation Rarely bite. RATS. Grab over neck and shoulders Place forefinger just below mandible on one side of head and thumb on opposite side either above or below forelimb

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RATS

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  1. RATS

  2. Usually very friendly and accept handling and gentle manipulation Rarely bite RATS

  3. Grab over neck and shoulders Place forefinger just below mandible on one side of head and thumb on opposite side either above or below forelimb Provide additional support by holding tail and hind limbs with opposite hand GENERAL RESTRAINT

  4. Scruffing Technique Same as in gerbils and mice Don not like it

  5. Wrap in thin towel or stockinette Syringe case with air hole Cone shaped polyethylene bag with tip cut off Full Restraint

  6. http://awic.nal.usda.gov/nal_display/index.php?info_center=3&tax_level=3&tax_subject=183&topic_id=1093&level3_id=6273&level4_id=0&level5_id=0&placement_default=0http://awic.nal.usda.gov/nal_display/index.php?info_center=3&tax_level=3&tax_subject=183&topic_id=1093&level3_id=6273&level4_id=0&level5_id=0&placement_default=0 http://www.bva-awf.org.uk/resources/tutorials/BVA02-Introduction.html

  7. Intraperitoneal injection Blood collection from tail vein Blood collection from cardiac puncture Oral feeding Chemical Restraint Laboratory Animal Handling Technique - Rat

  8. The most common method of administering drugs to rats Intraperitoneal Injection in Rat

  9. 75% alcohol cotton ball for surface disinfection medium size towel for restraining the rat 25G 5/8” needle with 1cc syringe for injection Tools for intraperitoneal injection in rat

  10. Let the rat relaxes on the top of the lid.

  11. Stretch the body of the rat by pulling up it’s tail and then cover the rat with a towel by your left hand

  12. Fold the skirts of towel under the rat from all directions

  13. Grasp up the left hindlimb of the rat to expose the abdomen

  14. The injection site should be in the lower left quadrant of the abdomen because vital organs are absent from this area

  15. Only the tip of the needle should penetrate the abdominal wall to prevent injection into the intestines.

  16. small amount: 0.1-1 ml Collection of blood from tail vein in rat

  17. 75% alcohol cotton ball for surface disinfection 27G1/2” needle with 1 ml syringe for blood withdrawal a vial for blood collection Tools for collection of blood from tail vein

  18. Optimal site of blood withdrawal is around the distal one-third of the tail since this part of tail gives better visualization of the veins

  19. The rat's tail has a thermoregulatory function: it serves as a heat-loss organ. The tail is well suited for this purpose, because it has no fur, has a large surface to volume ratio, and is perfused with many blood vessels, especially at the tail tip and midlength (Yulong et al. 1995).

  20. Temperature influence Rats control their body temperature through their tails by dilating or constricting their tail blood vessels.

  21. Disinfect the tail with 75% alcoholic cotton ball

  22. When the needle penetrates the epithelium of the tail, pull back the plugger a bit to create negative pressure inside the syringe, then push the needle in the vein slowly until blood get into the dead space of the needle head

  23. Pull back the plugger by the ring finger to withdraw blood from the tail vein

  24. Using a scalpel to make a small wound on the tail is also an option for collecting blood from tail vein

  25. Blood can be collected b using a pipetteman after then

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