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Electroporation of linear BAC. ori. Cm. Linearised BAC 2 ( hLhcgr ). HA2a. HA2b. ori. Sm. DSB. DSB. b. b. b. HA2b. HA2a. b. b. b. HA2a. HA2b. HA2b. HA2a. Rec a. Rec a. b. b. b. *. HA1a. RpsL-NEO. *. HA1b. HA2a. HA2b. ISce-I. gba. b. b. b.

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  1. Electroporation of linear BAC ori Cm Linearised BAC 2 (hLhcgr) HA2a HA2b ori Sm DSB DSB b b b HA2b HA2a b b b HA2a HA2b HA2b HA2a Reca Reca b b b * HA1a RpsL-NEO * HA1b HA2a HA2b ISce-I gba b b b BAC human Lhcgr gene into mouse Lhcgr BAC (hmLhcgr) Red/ET ISceI (amp) Red/ET and ISceI E.coli Electro-competent, Red/ET and I-SceI expressing E.coli

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